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Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat

Qiuhong Wu,  Yongxing Chen,  Beibei Li,  Jing Li,  Panpan Zhang,  Jingzhong Xie,  Huaizhi Zhang,  Guanghao Guo,  Ping Lu,  Miaomiao Li,  Keyu Zhu,  Wenling Li,  Tzion Fahima,  Eviatar Nevo,  Hongjie Li,  Lingli Dong,  Zhiyong Liu

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文章导航 > 机械工程学报  > 优先出版
Qiuhong Wu, Yongxing Chen, Beibei Li, Jing Li, Panpan Zhang, Jingzhong Xie, Huaizhi Zhang, Guanghao Guo, Ping Lu, Miaomiao Li, Keyu Zhu, Wenling Li, Tzion Fahima, Eviatar Nevo, Hongjie Li, Lingli Dong, Zhiyong Liu. Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat[J]. 机械工程学报. doi: 10.1016/j.jgg.2022.01.010
引用本文: Qiuhong Wu, Yongxing Chen, Beibei Li, Jing Li, Panpan Zhang, Jingzhong Xie, Huaizhi Zhang, Guanghao Guo, Ping Lu, Miaomiao Li, Keyu Zhu, Wenling Li, Tzion Fahima, Eviatar Nevo, Hongjie Li, Lingli Dong, Zhiyong Liu. Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat[J]. 机械工程学报. doi: 10.1016/j.jgg.2022.01.010
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Qiuhong Wu, Yongxing Chen, Beibei Li, Jing Li, Panpan Zhang, Jingzhong Xie, Huaizhi Zhang, Guanghao Guo, Ping Lu, Miaomiao Li, Keyu Zhu, Wenling Li, Tzion Fahima, Eviatar Nevo, Hongjie Li, Lingli Dong, Zhiyong Liu. Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat[J]. JOURNAL OF MECHANICAL ENGINEERING. doi: 10.1016/j.jgg.2022.01.010
Citation: Qiuhong Wu, Yongxing Chen, Beibei Li, Jing Li, Panpan Zhang, Jingzhong Xie, Huaizhi Zhang, Guanghao Guo, Ping Lu, Miaomiao Li, Keyu Zhu, Wenling Li, Tzion Fahima, Eviatar Nevo, Hongjie Li, Lingli Dong, Zhiyong Liu. Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat[J]. JOURNAL OF MECHANICAL ENGINEERING. doi: 10.1016/j.jgg.2022.01.010
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Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat

doi: 10.1016/j.jgg.2022.01.010

  • a. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China;

  • b. College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;

  • c. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, China;

  • d. Institute of Evolution and the Department of Evolutionary and Environmental Biology, University of Haifa, Mt. Carmel, Haifa 3498838, Israel;

  • e. The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

基金项目: 

We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China, for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China (31971876, U21A20224) and Scientific Research Project of Beijing Municipal Commission of Education (KM201910020014).

详细信息
    通讯作者:

    Lingli Dong,E-mail:lldong@genetics.ac.cn

    Zhiyong Liu,E-mail:zyliu@genetics.ac.cn

Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat

  • a. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China;

  • b. College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;

  • c. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, China;

  • d. Institute of Evolution and the Department of Evolutionary and Environmental Biology, University of Haifa, Mt. Carmel, Haifa 3498838, Israel;

  • e. The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Funds: 

We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China, for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China (31971876, U21A20224) and Scientific Research Project of Beijing Municipal Commission of Education (KM201910020014).

    摘要
  • 摘要: Wild emmer wheat (Triticum dicoccoides, WEW) is an immediate progenitor of both the cultivated tetraploid and hexaploid wheats and it harbors rich genetic diversity against powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). A powdery mildew resistance gene MlIW172 originated from WEW accession IW172 (G-797-M) is fine mapped in a 0.048 centimorgan (cM) genetic interval on 7AL, corresponding to a genomic region spanning 233kb, 1Mb and 800kb in Chinese Spring, WEW Zavitan, and T.urartu G1812, respectively. MlIW172 encodes a typical NLR protein NLRIW172 and physically locates in an NBS-LRR gene cluster. NLR is subsequently identified as a new allele of Pm60, and its function is validated by EMS mutagenesis and transgenic complementation. Haplotype analysis of the Pm60 alleles reveals diversifications in sequence variation in the locus and presence and absence variations (PAV) in WEW populations. Four common single nucleotide variations (SNV) are detected between the Pm60 alleles from WEW and T.urartu, indicative of speciation divergence between the two different wheat progenitors. The newly identified Pm60 alleles and haplotypes in WEW are anticipated to be valuable for breeding powdery mildew resistance wheat cultivars via marker-assisted selection.

     

    Abstract: Wild emmer wheat (Triticum dicoccoides, WEW) is an immediate progenitor of both the cultivated tetraploid and hexaploid wheats and it harbors rich genetic diversity against powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). A powdery mildew resistance gene MlIW172 originated from WEW accession IW172 (G-797-M) is fine mapped in a 0.048 centimorgan (cM) genetic interval on 7AL, corresponding to a genomic region spanning 233kb, 1Mb and 800kb in Chinese Spring, WEW Zavitan, and T.urartu G1812, respectively. MlIW172 encodes a typical NLR protein NLRIW172 and physically locates in an NBS-LRR gene cluster. NLR is subsequently identified as a new allele of Pm60, and its function is validated by EMS mutagenesis and transgenic complementation. Haplotype analysis of the Pm60 alleles reveals diversifications in sequence variation in the locus and presence and absence variations (PAV) in WEW populations. Four common single nucleotide variations (SNV) are detected between the Pm60 alleles from WEW and T.urartu, indicative of speciation divergence between the two different wheat progenitors. The newly identified Pm60 alleles and haplotypes in WEW are anticipated to be valuable for breeding powdery mildew resistance wheat cultivars via marker-assisted selection.

     

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  • 收稿日期:  2021-11-27
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