Antigenic and genetic characteristics of influenza B virus/Victoria lineage in Shanghai, 2018–2020
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摘要:目的 分析2018 — 2020年上海市分离的B/Victoria系流感毒株与疫苗株的匹配性及基因变异情况。方法 利用血凝抑制实验，对142株B/Victoria系流感毒株进行了抗原性分析，并选取了63株开展了血凝素（HA）及神经氨酸酶（NA）基因序列分析。结果 2018 — 2020年上海市流行的B/Victoria系流感毒株主要属于V1A.3分支，少数属于V1A.1分支及未缺失的V1A分支；抗原性分析显示，26.76%的流行株是疫苗株B/Colorado/06/2017鸡胚株的低反应株，与疫苗株相比，V1A.3分支流行株在HA蛋白的120环、150环、160环及190螺旋等关键的抗原决定簇及受体结合关键部位发生了5个氨基酸位点改变。结论 2018 — 2020监测年度B/Victoria系流行株与世界卫生组织推荐的疫苗株组分B/Colorado/06/2017匹配性不佳，仍需密切监测流行株的谱系及变异情况，为疫苗株的筛选提供可靠的数据。Abstract:Objective To analyze the antigenic and genetic characteristics and variation of influenza B virus /Victoria lineage in Shanghai during 2018–2020.Methods Hemagglutinin inhibition test was performed to analyze the antigenicity of 142 strains of influenza B virus/Victoria lineage isolated in Shanghai from April 2018 to March 2020, in which 63 strains were used for hemagglutinin and neuraminidase gene sequencing and analysis.Results V1A.3 was predominant clade in influenza B virus B/Victoria lineage circulating in Shanghai. A few circulating strains belonged to clade V1A.1 and clade V1A without deletion. Only 26.76% of the circulating virus strains were antigenically similar to the B/Colorado/06/2017 vaccine strain. Five amino acids loci had mutation in the HA protein sequence of clade V1A.3 compared with the B/Colorado/06/2017 vaccine strain, involving 4 important epitopes and receptors, such as 120 loop, 150 loop, 160 loop and 190 helix.Conclusion The majority of influenza B virus /Victoria lineage circulating in Shanghai did not match well with the vaccine strain B/Colorado/06/2017 recommended by WHO. It is necessary to further strengthen the surveillance for influenza virus variation to provide reliable data for the screening of influenza vaccine strain.
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Key words:
- Influenza virus B/Victoria lineage /
- Hemagglutimin /
- Neuraminidase /
- Antigenicity /
- Genetic characteristics /
- t /
- c
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图 1 2018－2020年上海市B/Victoria系流感病毒real-time RCR鉴定阳性病例
Figure 1. Cases of influenza B virus/Victoria lineage infection identified by real time PCR in Shanghai, 2018–2020
图 2 2018－2020年上海市B/Victoria系流感病毒感染人群年龄分布
Figure 2. Age distribution of people infected with influenza B virus/Victoria lineage in Shanghai, 2018–2020
图 3 2018－2020年监测年度上海市分离的B/Victoria系流感病毒HA基因进化树
注：
. 2009—2012、2013—2018年度疫苗株；●. 2018—2020年度疫苗株；▲. 2020—2021年度疫苗株 Figure 3. Phylogenetic tree of HA genes of influenza B virus/Victoria lineage isolated in Shanghai, 2018–2020
图 4 2018－2020年监测年度上海市分离的B/Victoria系流感病毒NA基因进化树
注：
. 2009—2012、2013—2018年度疫苗株；●. 2018—2020年度疫苗株；▲. 2020—2021年度疫苗株 Figure 4. Phylogenetic tree of NA genes of influenza B virus/Victoria lineage isolated in Shanghai, 2018–2020
表 2 2018－2020年监测年度上海市分离的B/Victoria系流感病毒HA蛋白位点变异情况
Table 2. Mutation sites of amino acid of HA genes of influenza B virus/Victoria lineage isolated in Shanghai, 2018–2020
分支 位点 B/Colorado/06/2017 氨基酸变异情况 是否属于抗原决定簇 是否属于受体结合部位 是否属于糖基化位点 V1A.3 133 G G133R 120环 否 否 V1A.3 136 K K136E 120环 140环 否 V1A.3 164 D 缺失 160环 否 否 V1A.3 180 V V180I 否 否 否 V1A.3 197 T T197N/T197S 190螺旋 190螺旋 增加NETQ V1A.3 498 K K498R 否 否 否 V1A.3 184 G G184E 否 否 否 V1A.3 252 V V252M 否 否 否 V1A.3 279 R R279K 否 否 否 V1A 129 G G129D 120环 否 否 V1A 162 K 不缺失 160环 否 否 V1A 163 N 不缺失 160环 否 否 V1A 180 V V180I 否 否 否 V1A 197 T T197N/T197S 190螺旋 190螺旋 增加NETQ V1A 498 K K498R 否 否 否 
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