Volume 40 Issue 5
May. 2023
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TANG Jing-hui, QIN Man-man, TANG Le, WEI Hua, QIU Liang, YU Jun. Lactobacillus plantarum ZDY04 reduces Trimethylamine N-Oxide-induced atherosclerosis by promoting reverse cholesterol transport in ApoE-/- mice[J]. JOURNAL OF MECHANICAL ENGINEERING, 2023, 1(5): 24-34. doi: 10.13381/j.cnki.cjm.00000-00
Citation: TANG Jing-hui, QIN Man-man, TANG Le, WEI Hua, QIU Liang, YU Jun. Lactobacillus plantarum ZDY04 reduces Trimethylamine N-Oxide-induced atherosclerosis by promoting reverse cholesterol transport in ApoE-/- mice[J]. JOURNAL OF MECHANICAL ENGINEERING, 2023, 1(5): 24-34. doi: 10.13381/j.cnki.cjm.00000-00

Lactobacillus plantarum ZDY04 reduces Trimethylamine N-Oxide-induced atherosclerosis by promoting reverse cholesterol transport in ApoE-/- mice

doi: 10.13381/j.cnki.cjm.00000-00
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  • Corresponding author: QIU Liang, E-mail: liangqiu@jxutcm.edu.cn
  • Received Date: 22 Dec 2020
  • Rev Recd Date: 24 Mar 2021
  • Available Online: 19 Sep 2022
  • Issue Publish Date: 31 May 2023
  • Objective  To observe the effect of Lactobacillus plantarum ZDY04 in Trimethylamine N-Oxide (TMAO)-induced atherosclerosis in mice and its underlying mechanisms.
    Methods  Thirty female (six to eight-weeks old) apolipoprotein E-deficient (ApoE-/-) mice were randomly assigned into three groups as follows: Chow group, Choline+PBS group and Choline+ZDY04 group, and fed with 1.3% high-choline diet to establish atherosclerosis models, then treated with sterile PBS or ZDY04 containing 15% glycerol for 16 weeks. After treatment, serum lipids were measured using cholesterol kit. The serum TMAO and fecal TMA content were determined by using liquid chromatography-tandem mass spectrometry LC-MS. Atherosclerotic lesion formation in whole aorta was detected with Oil red O staining. Hematoxylin-eosin, Oil red O and Masson staining were used to detect the atherosclerotic area, lipid deposition and collagen content in the aortic roots. Immunofluorescent staining of macrophage specific antigen CD68 and smooth muscle a-actin (SMA) were used to examine the lesion composition in the aortic root. The expression of genes related to reverse cholesterol transport and Fmo3 in liver was detected with RT-qPCR and Western blot.
    Results  Compared with Choline+PBS group, the atherosclerotic lesions in the whole aortas significantly reduced in Choline+ZDY04 group. ZDY04 treatment significantly decreased atherosclerotic lesion size, lipid deposition and macrophage content, but not the contents of lesion smooth muscle cells or collagen, when compared to Choline+PBS fed mice. The content of cecum TMA and serum TMAO decreased significantly in Choline+ZDY04 group; serum high density lipoprotein-cholesterol significantly increased, but total cholesterol and low density lipoprotein-cholesterol only marginally decreased. ZDY04 significantly increased the mRNA expression of reverse cholesterol transport genes Sr-b1, Abcg5, Cyp7a1 but not Fmo3. Liver CYP7A1 protein level was also increased by ZDY04 compared to controls.
    Conclusion  Lactobacillus plantarum ZDY04 can inhibit TMAO induced thermogenesis by promoting reverse transport and decomposition of cholesterol in ApoE-/- mice.

     

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