A Preliminary on the Bactericidal Mechanism of Slightly Acidic Electrolyzed Water on <i>Salmonella enteritidis</i>

SUN Lina; LI Yuwei; LIU Changlin; YUAN Xingyun; WANG Yuchen; JIA Yingqi; ZANG Yitian; MO Qingnan; ZHANG Beibei; ZHENG Jiahui; ZHANG Ran; SHU Dengqun

doi:10.13386/j.issn1002-0306.2022060100

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May. 2023

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JOURNAL OF MECHANICAL ENGINEERING > 2023 > 44(9): 146-152.

SUN Lina, LI Yuwei, LIU Changlin, et al. A Preliminary on the Bactericidal Mechanism of Slightly Acidic Electrolyzed Water on Salmonella enteritidis[J]. Science and Technology of Food Industry, 2023, 44(9): 146−152. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060100

Citation:

SUN Lina, LI Yuwei, LIU Changlin, et al. A Preliminary on the Bactericidal Mechanism of Slightly Acidic Electrolyzed Water on Salmonella enteritidis[J]. Science and Technology of Food Industry, 2023, 44(9): 146−152. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060100

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A Preliminary on the Bactericidal Mechanism of Slightly Acidic Electrolyzed Water on Salmonella enteritidis

doi: 10.13386/j.issn1002-0306.2022060100

1.
Nanchang Key Laboratory of Animal Health and Safety, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China
2.
Nanchang Academy of Agricultural Sciences, Nanchang 330299, China

Received Date: 14 Jun 2022
Issue Publish Date: 01 May 2023

Abstract

Abstract

In order to study the bactericidal mechanism of slightly acidic electrolyzed water (SAEW) against Salmonella enteritidis, the effects of SAEW on cell membrane permeability, cell membrane integrity, reactive oxygen species (ROS), and intracellular ATP of Salmonella enteritidis were studied. The results showed that 2.04 lg (CFU/g) of Salmonella enteritidis on the surface of eggshells was inactivated after the SAEW treatment at the available chlorine concentration of 10 mg/L for 1 min. Moreover, relative to the control group, the conductivity of bacterial suspension of Salmonella enteritidis was increased from 13.78±0.13 ms/cm to 16.32±0.10 ms/cm (P<0.05) in the SAEW group. In addition, the protein leakage in the bacterial cells was significantly increased from 0.07±0.04 g prot/L to 0.30±0.03 g prot/L (P<0.05). Moreover, the SAEW treatment increased the nucleic acid leakage and ROS accumulation and the intracellular ATP content reduction. The result of SEM also showed that, after the SAEW treatment, the cell wall structure of Salmonella enteritidis was incomplete, damaged and obviously perforated, and many bacterial surfaces were sunken, deformed or broken and adhered. The results showed that SAEW significantly increased the permeability and seriously destroyed the integrity of the cell membranes of Salmonella enteritidis, which led to the leakage of intracellular proteins, nucleic acids, ATP and other contents, leading to the accumulation of ROS.
- slightly acidic electrolyzed water,
- Salmonella enteritidis,
- sterilization mechanism

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References

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A Preliminary on the Bactericidal Mechanism of Slightly Acidic Electrolyzed Water on Salmonella enteritidis

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