Issue 9
May. 2023
Turn off MathJax
Article Contents
LI Yuanda, WU Ting, HUANG Ganghua, et al. SPME-GC-MS Technique Combined with rOAV for the Analysis of Key Aroma Substances of Zijuan White Tea with Different Processing Processes[J]. Science and Technology of Food Industry, 2023, 44(9): 324−332. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060240
Citation: LI Yuanda, WU Ting, HUANG Ganghua, et al. SPME-GC-MS Technique Combined with rOAV for the Analysis of Key Aroma Substances of Zijuan White Tea with Different Processing Processes[J]. Science and Technology of Food Industry, 2023, 44(9): 324−332. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060240

SPME-GC-MS Technique Combined with rOAV for the Analysis of Key Aroma Substances of Zijuan White Tea with Different Processing Processes

doi: 10.13386/j.issn1002-0306.2022060240
  • Received Date: 27 Jun 2022
  • Issue Publish Date: 01 May 2023
  • In order to study the key aroma compounds of Zijuan white tea with different processing technologies, headspace solid-phase micro extraction and gas chromatography mass spectrometry (HS-SPME-GC-MS), combined with sensory evaluation, PCA validation, OPLS-DA analysis and relative odor activity value (rOAV), was used to analyze and screen the key aroma compounds of Zijuan white tea. The results showed that 82 major aroma components were detected, mainly alcohols, esters, heterocyclic and aromatic compounds, with relatively high contents of linalool, methyl salicylate, benzyl alcohol and β-Ionone. The results of rOAV analysis showed that 9 volatile organic compounds including 1-octen-3-ol, α-Ionone, phenylacetaldehyde and methyl salicylate contributed to the aroma formation of natural withering Zijuan white tea (TZW) with floral and fruity aroma and spicy medicinal aroma, and 9 volatile organic compounds including 1-octen-3-ol, α-Ionone, β-Ionone and benzaldehyde contributed to the aroma formation of compound withering Zijuan white tea (CZW) with floral and fruity aroma and spicy aroma. OPLS-DA analysis combined with rOAV showed that 1-octene-3-ol and methyl salicylate were the key compounds for aroma difference between TZW and CZW. In summary, the flower and fruit aroma of Zijuan white tea processed by natural wilting was more abundant, which could effectively improve the aroma quality of Zijuan white tea.

     

  • loading
  • [1]
    赵瑜, 周家春, 张靖伟, 等. 紫娟茶提取物对血管紧张素转换酶、α-淀粉酶和胰脂肪酶的体外抑制作用[J]. 食品工业科技,2017,38(19):11−20. [ZHAO Y, ZHOU J C, ZHANG J W, et al. In vitro inhibition of angiotensin-converting enzyme, α-amylase and pancreatic lipase by Zijuan tea extract[J]. Food Industry Technology,2017,38(19):11−20.
    [2]
    南占东, 季鹏章, 农国富, 等. 云南特有茶树资源紫娟生物活性物质研究[J]. 食品科技,2013,38(11):229−231. [NAN Z D, JI P Z, NONG G F, et al. Study on bioactive substances of Zijuan, an endemic tea tree resource in Yunnan[J]. Food Technology,2013,38(11):229−231.
    [3]
    张维成. 云南紫娟特种茶特征及栽培要点[J]. 中国茶叶,2013,35(1):25. [ZHANG W C. Characteristics and cultivation points of Zijuan special tea in Yunnan[J]. China Tea,2013,35(1):25. doi: 10.3969/j.issn.1000-3150.2013.01.011
    [4]
    陈荣达. 紫娟茶花青素抑制酪氨酸酶活性及其作用机制研究[D]. 吉林: 长春师范大学, 2021

    CHEN R D. Inhibition of tyrosinase activity by anthocyanins from Zijuan tea and its mechanism of action[D]. Jilin: Changchun Normal University, 2021.
    [5]
    WANG D, XIE D, LU M, et al. Characterization of white tea metabolome: Comparison against green and black tea by a nontargeted metabolomics approach[J]. Food Res Int,2017:9640−45.
    [6]
    翁开振, 翁丽娟, 吴加旺. 福鼎白茶加工关键技术研究[J]. 现代食品,2019(17):50−52. [WENG K Z, WENG L J, WU J W. Research on key technology of Fuding white tea processing[J]. Modern Food,2019(17):50−52.
    [7]
    陈可坚. 不同萎凋方式对白茶品质的影响[J]. 福建茶叶,2020,42(6):35−36. [CHEN K J. Effect of different withering methods on the quality of white tea[J]. Fujian Tea,2020,42(6):35−36. doi: 10.3969/j.issn.1005-2291.2020.06.019
    [8]
    谢文钢, 范仕胜, 刘燕, 等. HS-SPME/GC-MS分析“乌牛早”绿茶香气[J]. 茶叶通讯,2019,46(4):448−454. [XIE W G, FAN S S, LIU Y, et al. HS-SPME/GC-MS analysis of the aroma of "Wu Niu Zao" green tea[J]. Tea Newsletter,2019,46(4):448−454. doi: 10.3969/j.issn.1009-525X.2019.04.012
    [9]
    XUE J, GUO G, LIU P, et al. Identification of aroma-active compounds responsible for the floral and sweet odors of Congou black teas using gas chromatography-mass spectrometry/olfactometry, odor activity value, and chemometrics[J]. J Sci Food Agric, 2022, 102(12).
    [10]
    GUO X, SCH W, HO C T, et al. Characterization of the aroma profiles of oolong tea made from three tea cultivars by both GC-MS and GC-IMS[J]. Food Chem,2021:376131933.
    [11]
    CHEN S, TANG J, FAN S, et al. Comparison of potent odorants in traditional and modern types of Chinese xiaoqu liquor (Baijiu) based on odor activity values andmultivariate analyses[J]. Foods,2021,10(10):2392. doi: 10.3390/foods10102392
    [12]
    DENG X J, HUANG G, TU Q, et al. Evolution analysis of flavor-active compounds during artificial fermentation of Pu-erh tea[J]. Food Chem,2021:357129783.
    [13]
    GUO X, HO C T, WAN X, et al. Changes of volatile compounds and odor profiles in Wuyi rock tea during processing[J]. Food Chem,2021,341(1):128230.
    [14]
    苏丹, 黄刚骅, 李亚莉, 等. 紫娟(熟茶)人工发酵过程中挥发性香气组分特征分析[J]. 食品科学,2021,42(12):166−172. [SU D, HUANG G H, LI Y L, et al. Characterization of volatile aroma components during the artificial fermentation of Zijuan (ripe tea)[J]. Food Science,2021,42(12):166−172. doi: 10.7506/spkx1002-6630-20200528-347
    [15]
    杨雪梅, 任洪涛, 罗琼仙, 等. ‘紫娟’红茶和‘紫娟’普洱熟茶香气成分的分析[J]. 热带农业科学,2017,37(5):72−82. [YANG X M, REN H T, LUO Q X, et al. Analysis of aroma components of 'Zijuan' black tea and 'Zijuan' ripe pu-erh tea[J]. Tropical Agricultural Science,2017,37(5):72−82.
    [16]
    陈保, 姜东华, 罗发美, 等. 四种不同加工工艺紫娟茶香气成分的比较[J]. 现代食品科技,2013,29(10):2480−2486. [CHEN B, JIANG D H, LUO F M, et al. Comparison of aroma composition of four different processing processes of Zijuan tea[J]. Modern Food Science and Technology,2013,29(10):2480−2486.
    [17]
    国家质量监督检验检疫总局. 国家标准化管理委员会. GB/T 23776-2018 茶叶感官审评方法[S].

    General Administration of Quality Supervision. Inspection and Quarantine of the People’s Republic of China Standardization Administration. GB/T 23776-2018 Sensory evaluation method of tea[S].
    [18]
    国家质量监督检验检疫总局. 国家标准化管理委员会. GB/T 22291-2017 白茶[S].

    General Administration of Quality Supervision. Inspection and Quarantine of the People’s Republic of China Standardization Administration. GB/T 22291-2017 White tea[S].
    [19]
    FENG M, DAI Z, YIN Z, et al. The volatile flavor compounds of Shanghai smoked fish as a special delicacy[J]. J Food Biochem,2021,45(1):e13553.
    [20]
    QIN C C, ZHU Y, YAN H, et al. Identification of aroma composition and key odorants contributing to aroma characteristics of white teas[J]. Molecules (Basel, Switzerland), 2020, 21(24).
    [21]
    吴婷, 邓秀娟, 李沅达, 等. 云茶香1号不同萎凋工艺白茶的化学品质研究[J]. 食品安全质量检测学报,2021,12(24):9530−9538. [WU T, DENG X J, LI Y D, et al. Research on chemical quality of white tea with different withering processes of Yuncha Xiang No.1[J]. Journal of Food Safety and Quality Inspection,2021,12(24):9530−9538. doi: 10.3969/j.issn.2095-0381.2021.24.spaqzljcjs202124029
    [22]
    高夏洁, 高海燕, 赵镭, 等. SPME-GC-MS结合OAV分析不同产区花椒炸花椒油的关键香气物质[J]. 食品科学,2022,43(4):208−214. [GAO X J, GAO H Y, ZHAO L, et al. Analysis of key aroma substances in fried pepper oil from different production areas by SPME-GC-MS combined with OAV[J]. Food Science,2022,43(4):208−214. doi: 10.7506/spkx1002-6630-20201120-217
    [23]
    XIE B, WU Q, WEI S, et al. Optimization of headspace solid-phase micro-extraction conditions (HS-SPME) and identification of major volatile aroma-active compounds in Chinese chive (Allium tuberosum Rottler)[J]. Molecules, 2022, 27(8): 8.
    [24]
    BI J, LI Y, YANG Z, et al. Effect of different cooking times on the fat flavor compounds of pork belly[J]. J Food Biochem,2022:e14184.
    [25]
    石亚丽, 朱荫, 马婉君, 等. 名优炒青绿茶挥发性成分研究进展[J]. 茶叶科学,2021,41(3):285−301. [SHI Y L, ZHU M, MA W J, et al. Research progress on volatile components of famous green tea[J]. Tea Science,2021,41(3):285−301. doi: 10.3969/j.issn.1000-369X.2021.03.001
    [26]
    降升平, 张小红, 张玲玲, 等. 4个品种茶叶的香气成分比较[J]. 食品研究与开发,2013,34(15):66−70. [JIANG S P, ZHANG X H, ZHANG L L, et al. Comparison of aroma composition of four varieties of tea[J]. Food Research and Development,2013,34(15):66−70. doi: 10.3969/j.issn.1005-6521.2013.15.018
    [27]
    黄刚骅, 李沅达, 邓秀娟, 等. 四种干燥方式云南白茶的香气组分分析[J]. 食品工业科技,2022,43(18):283−29. [HUANG G H, LI Y D, DENG X J, et al. Analysis of aroma components of Yunnan white tea by four drying methods[J]. Food Industry Science and Technology,2022,43(18):283−29.
    [28]
    陈志达, 温欣黎, 陈兴华, 等. 不同等级福鼎白茶香气成分研究[J]. 浙江大学学报(农业与生命科学版),2019,45(6):715−722. [CHEN Z D, WEN X L, CHEN X H, et al. Study on the aroma composition of different grades of Fuding white tea[J]. Journal of Zhejiang University (Agriculture and Life Science Edition),2019,45(6):715−722. doi: 10.3785/j.issn.1008-9209.2019.02.261
    [29]
    沈强, 张小琴, 刘晓霞, 等. 不同阶段性返白过程正安白茶香气成分分析[J]. 食品科技,2021,46(3):276−282. [SHEN Q, ZHANG X Q, LIU X X, et al. Analysis of aroma components of Zheng'an white tea during different stages of rewhitening[J]. Food Science and Technology,2021,46(3):276−282.
    [30]
    林燕萍, 黄毅彪, 张渤, 等. 梅占红茶、白茶品质差异分析[J]. 食品工业科技,2022,43(3):260−267. [LIN Y P, HUANG, Y B, ZHANG B, et al. Analysis of quality differences between Meizhan black tea and white tea[J]. Food Industry Science and Technology,2022,43(3):260−267. doi: 10.13386/j.issn1002-0306.2021050222
    [31]
    王飞权, 冯花, 葛捷琳, 等. 不同茶树品种新工艺秋季白茶品质比较分析[J]. 茶叶通讯,2019,46(4):441−447. [WANG F Q, FENG H, GE J L, et al. Comparative analysis of the quality of autumn white tea with new process of different tea tree varieties[J]. Tea Communications,2019,46(4):441−447. doi: 10.3969/j.issn.1009-525X.2019.04.011
    [32]
    王志霞, 苏丹, 任洪涛, 等. 紫娟与丹妃挥发性成分差异分析[J]. 食品安全质量检测学报,2021,12(18):7388−7396. [WANG Z X, SU D, REN H T, et al. Analysis of the differences in volatile components between Zijuan and Danfei[J]. Journal of Food Safety and Quality Testing,2021,12(18):7388−7396.
    [33]
    里奥·范海默特. 化合物嗅觉阈值汇编[M]. 上海: 科学出版社, 2018: 1−229

    LEO VAN HEMER. Compilations of flavour thershold values in water and other media[M]. Shanghai: Science Press, 2018: 1−229.
  • 加载中

Catalog

    Figures(3)  / Tables(3)

    Article Metrics

    Article views(22) PDF downloads(0) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return