Optimization of Preparation Process of Collagen from <i>Takifugu Obscurus</i> Skin and Its Functional Properties

XU Xiao; XU Hui; TAO Ningping

doi:10.13386/j.issn1002-0306.2022070142

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May. 2023

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JOURNAL OF MECHANICAL ENGINEERING > 2023 > 44(10): 168-176.

XU Xiao, XU Hui, TAO Ningping. Optimization of Preparation Process of Collagen from Takifugu Obscurus Skin and Its Functional Properties[J]. Science and Technology of Food Industry, 2023, 44(10): 168−176. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070142

Citation:

XU Xiao, XU Hui, TAO Ningping. Optimization of Preparation Process of Collagen from Takifugu Obscurus Skin and Its Functional Properties[J]. Science and Technology of Food Industry, 2023, 44(10): 168−176. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070142

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Optimization of Preparation Process of Collagen from Takifugu Obscurus Skin and Its Functional Properties

doi: 10.13386/j.issn1002-0306.2022070142

XU Xiao^1
,,
XU Hui²,
TAO Ningping^{3,*
,
,}

1.
Jiangsu Zhongyang Group Co., Ltd., Development and Protection Engineering Technology Research Center of Yangtze Rare Fish, Haian 226600, China
2.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
3.
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China

Received Date: 14 Jul 2022
Issue Publish Date: 15 May 2023

Abstract

Abstract

The skin of Takifugu obscurus is high in collagen. There is a growing trend to study the preparation method and functional properties of fish skin collagen due to the additive values. This research aimed to optimize the collagen extraction processing from the skin of dark-striped pufferfish including the effects of pepsin enzyme addition, enzymatic hydrolysis time, ultrasonic power and ultrasonic time. The dextran gel column chromatography method was used to separate and identify the extracted and salt-desorized collagen. The inhibitory activity of extracted collagen on tyrosinase and hyaluronase was studied. Results showed that, the best extraction rate of collagen was 71.6%±2.7% at pepsin 4.2%, enzymatic hydrolysis time 38 h, ultrasonic power 320 W, and the ultrasonic time 9 mins. The inhibition rate of tyrosinase and hyaluronidase was 85% and 90% respectively, at concentration of Takifugu obscurus skin collagen was 0.8 mg/mL and the pH was 5.5, which performed better than the commercial fish skin collagen. This study indicated that the skin collagen of Takifugu obscurus had the great potential in whitening and anti-inflammatory application, which could be further applied in the field of cosmetics.
- Takifugu obscurus,
- collagen,
- extraction,
- purification,
- tyrosinase,
- hyaluronidase

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References(26)

References

[1]	农业部渔业渔政管理局. 中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2021. Fisheries and Fishery Administration of the Ministry of Agriculture. China fisheries statistical yearbook [M]. Beijing: China Agricultural Press, 2021.
[2]	王纯纯, 乔琨, 陈贝, 等. 河豚毒素的性质及应用研究进展[J]. 渔业研究,2021,43(5):539−548. [WANG C C, QIAO K, CHEN B, et al. Progress in the study of the nature and application of tetrodotoxin[J]. Fisheries Research,2021,43(5):539−548.
[3]	ZHANG J, ZOU M, ZHANG M, et al. Impact of telopeptides on self-assembly properties of snakehead (Channa argus) skin collagen[J]. Food Biophysics,2016(11):380−387.
[4]	刘永峰, 刘毅, 张振兴, 等. 鱼胶原蛋白提取方法研究进展[J]. 食品工业,2013,34(12):206−210. [LIU Y F, LIU Y, ZHANG Z X, et al. Research progress of fish collagen extraction method[J]. Food Industry,2013,34(12):206−210.
[5]	KOTCH F W, RONALD T R. Self-assembly of synthetic collagen triple helices[J]. P Natl Acad Sci,2006(9):3028−3033.
[6]	张建忠, 安辛欣, 王林, 等. 草鱼皮酸溶性和酶溶性胶原蛋白的提取及性质[J]. 食品科学,2006,27(12):556−559. [ZHANG J Z, AN X X, WANG L, et al. Extraction and properties of acid-soluble and enzyme-soluble collagen from grass carp skin[J]. Food Science,2006,27(12):556−559. doi: 10.3321/j.issn:1002-6630.2006.12.139
[7]	周瑞, 李若男, 周丽莎, 等. 暗纹东方鲀鱼皮胶原蛋白的提取及其特性[J]. 水产学报,2020,44(8):1349−1359. [ZHOU R, LI R N, ZHOU L S, et al. Extraction and characteristics of collagens from the skin of puffer fish[J]. Journal of Aquaculture,2020,44(8):1349−1359.
[8]	王晴, 钱玉梅, 李红侠, 等. 罗非鱼鱼皮胶原蛋白酶法提取工艺及特性研究[J]. 攀枝花学院学报,2020,37(5):6−10. [WANG Q, QIAN Y M, LI H X, et al. Study on the enzymatic extraction process and characteristics of collagen from non-fish skin[J]. Journal of Panzhihua College,2020,37(5):6−10.
[9]	JRIDI M, NASRI R, LASSOUED I, et al. Chemical and biophysical properties of gelatins extracted from alkali-pretreated skin of cuttlefish (Sepia officinalis) using pepsin[J]. Food Research International, 2013, 54(2): 1680-1687.
[10]	严秋萍, 邬应龙, 李月娥, 等. 齐口裂腹鱼骨胶原蛋白超声波辅助提取工艺及其特性研究[J]. 食品与机械,2017,33(12):154−159. [YAN Q P, WU Y L, LI Y E, et al. Ultrasonic-assisted extraction process and its characteristics of collagen from fish collagen[J]. Food and Machinery,2017,33(12):154−159.
[11]	万春燕, 王英华, 邬元娟, 等. 胶原蛋白在食品中的应用现状及其发展前景[J]. 中国食物与营养,2008(9):24−26. [WAN C Y, WANG Y H, WU Y J, et al. Current status of collagen application in food and its development prospect[J]. China Food and Nutrition,2008(9):24−26.
[12]	乐彩虹, 陶宁萍, 徐逍. 暗纹东方鲀鱼皮胶原蛋白肽脱苦前后苦味物质的变化[J]. 食品与发酵工业,2021,47(4):87−95. [LE C H, TAO N P, XU X, et al. Changes in bitter substances of collagen peptides from the skin of the dark oriental fish, P. orientalis, before and after de bittering[J]. Food and Fermentation Industry,2021,47(4):87−95.
[13]	ARUMUGAM G, SHARMA D, BALAKRISHNAN R M, et al. Extraction, optimization and characterization of collagen from sole fish skin[J]. Sustainable Chemistry & Pharmacy,2018,9:19−26.
[14]	王镜岩. 生物化学（上册）[M]. 北京: 高等教育出版社, 2002. WANG J Y. Biochemistry (upper volume) [M]. Beijing: Higher Education Press, 2002.
[15]	闫鸣艳. 狭鳕鱼皮胶原蛋白结构和物理特性的研究[D]. 青岛: 中国海洋大学, 2009. YAN M Y. Study on the structural and physical properties of collagen from pollock skin[D]. Qingdao: Ocean University of China, 2009.
[16]	SMIRNOFF N, CUMBES Q J. Hydroxyl radical scavenging activity of compatible solutes[J]. Phytochemistry,1989,28(4):1057−1060.
[17]	管雪娇, 邓尚贵. 鱿鱼皮营养成分分析[J]. 安徽农业科学,2013,41(27):11135−11137. [GUAN X J, DENG S G. Nutritional composition analysis of squid skin[J]. Anhui Agricultural Science,2013,41(27):11135−11137. doi: 10.3969/j.issn.0517-6611.2013.27.094
[18]	黄丹丹, 马良, 韩霜, 等. 超声预处理影响金枪鱼皮胶原酶解工艺及机理初探[J]. 食品与发酵工业,2017,43(4):141−146. [HUANG D D, MA L, HAN S, et al. A preliminary investigation on the process and mechanism of ultrasonic pretreatment affecting collagen digestion of tuna skin[J]. Food and Fermentation Industry,2017,43(4):141−146.
[19]	杨树奇, 曾少葵, 周春霞, 等. 3种鱼皮的基本成分及氨基酸组成分析[J]. 广东海洋大学学报,2010,30(1):97−100. [YANG S Q, ZENG S K, ZHOU C X, et al. Analysis of the basic composition and amino acid composition of three fish skin species[J]. Journal of Guangdong Ocean University,2010,30(1):97−100. doi: 10.3969/j.issn.1673-9159.2010.01.019
[20]	于浩. 鹿骨胶原蛋白的制备及抗氧化活性研究[D]. 长春: 长春工业大学, 2016. YU H. Preparation and antioxidant activity of deer bone collagen[D]. Changchun: Changchun University of Technology, 2016.
[21]	荆卉, 孙俊, 牟瑶瑶, 等. 超声波物化效应及其对蛋白质改性的应用研究进展[J]. 中国食品学报,2021,21(6):321−330. [JING H, SUN J, MOU Y Y, et al. Advances in ultrasonic physicochemical effects and their application to protein modification[J]. Chinese Journal of Food,2021,21(6):321−330.
[22]	史睿, 何静, 吉日木图. 超声波辅助提取骆驼皮胶原蛋白的工艺及结构表征[J]. 中国食品学报,2022,22(2):213−223. [SHI R, HE J, JI R M T, et al. Ultrasonic-assisted extraction of camel skin collagen and structural characterization[J]. Chinese Journal of Food,2022,22(2):213−223.
[23]	王锡念, 徐志善, 孙钦军, 等. 超声波辅助酶解提取鮟鱅鱼皮胶原蛋白的工艺优化[J]. 食品工业科技,2019,40(1):175−180. [WANG X N, XU Z S, SUN Q J, et al. Process optimization of ultrasound-assisted enzymatic extraction of collagen from monkfish skin[J]. Food Industry Science and Technology,2019,40(1):175−180.
[24]	YOU J K, NO J K, JI H L, et al. 4,4'-Dihydroxybiphenyl as a new potent tyrosinase Inhibitor[J]. Biological and Pharmaceutical Bulletin,2005,28(2):323−327. doi: 10.1248/bpb.28.323
[25]	郭海阳, 莫林兰, 谭海生, 等. 茶皂素的美白功效及其抑菌和抗氧化活性研究[J]. 中国粮油学报,2020,35(6):83−89. [GUO H Y, MO L L, TAN H S, et al. Study on the whitening efficacy of tea saponins and their antibacterial and antioxidant activities[J]. Chinese Journal of Grain and Oil,2020,35(6):83−89. doi: 10.3969/j.issn.1003-0174.2020.06.013
[26]	钟秋琴. 葛根美白活性物质的分离提取及在化妆品中的应用[D]. 无锡: 江南大学, 2008. ZHONG Q Q. Isolation and extraction of whitening active substances from pueraria mirifica and their application in cosmetics[D]. Wuxi: Jiangnan University, 2008.

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