Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology

CHEN Shaoshu; DONG Liting; XIA Fengteng; LIU Yujie; LUO Can; LI Yicheng; WANG Zheng

doi:10.13386/j.issn1002-0306.2022070321

Issue 9

May. 2023

Turn off MathJax

Article Contents

JOURNAL OF MECHANICAL ENGINEERING > 2023 > 44(9): 200-206.

CHEN Shaoshu, DONG Liting, XIA Fengteng, et al. Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology[J]. Science and Technology of Food Industry, 2023, 44(9): 200−206. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070321

Citation:

PDF( 2673 KB)

Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology

doi: 10.13386/j.issn1002-0306.2022070321

1.
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
2.
Hunan Imagination Agricultural Science and Technology, Zhangjiajie 427000, China

Received Date: 02 Aug 2022
Issue Publish Date: 01 May 2023

Abstract

Abstract

Discarded eggshells and wet soybean dregs are rich sources of calcium and nitrogen. This study aimed to establish a novel optimized method for the utilization of agricultural wastes. Eggshells and soybean dregs were the primary raw materials used in this study. Bacillus subtilis, Lactobacillus plantarum, and Enterococcus faecalis were the probiotic strains selected for mixed fermentation. Harman’s single-factor test based on the assessment of calcium lactate content in the fermented forage was used to screen the following process parameters: Ratio of soybean dregs to eggshells, solid to water ratio, addition of glucose, inoculation amount, ratio of probiotic strains, fermentation temperature and duration. Optimum fermentation conditions were determined using response surface methodology. The results revealed that the conditions required for optimum fermentation were as follows: Soybean dregs to eggshells ratio of 90:10, solid to water ratio of 1:2.5, addition of 15.6% glucose, inoculation amount of 14.7%, Bacillus subtilis, Lactobacillus plantarum and Enterococcus faecalis in the ratio of 1:1:1 (v/v), and fermentation duration of 6 days at 37 °C. The amount of calcium lactate in the fermented forage was 13.58%. Free amino acid and calcium lactate concentrations in the fermented forage were significantly higher (P<0.05) in comparison to their concentrations in the raw materials. A significant decrease in pH and concentrations of crude fat and soluble protein in the fermented forage was also observed (P<0.05). In conclusion, this study established a novel optimized method for the production of high calcium feed from eggshells and soybean dregs using probiotic fermentation.
- soybean dregs,
- eggshells,
- solid fermentation,
- calcium lactate,
- high calcium feed

FullText(HTML)

References(26)

References

[1]	刘颖, 李述刚, 李秀婷, 等. 新冠疫情防控常态化背景下中国禽蛋产业发展的问题及对策[J]. 食品与机械,2021,37(6):224−228. [LIU Y, LI S G, LI X T, et al. Problems and countermeasures on the development of poultry egg industry in China under the normalized background of COVID-19 control and prevention[J]. Food and Machinery,2021,37(6):224−228. doi: 10.13652/j.issn.1003-5788.2021.06.037
[2]	CHENG H J, WEI J L, LIANG M, et al. Calcium glycerolate catalyst derived from eggshell waste for cyclopentadecanolide synthesis[J]. Front Chem,2021,9:770247. doi: 10.3389/fchem.2021.770247
[3]	童玲, 罗桂林, 姜文娟, 等. 废弃蛋壳综合利用制备丙酸钙工艺研究[J]. 山东化工,2021,50(21):49−50, 53. [TONG L, LUO G L, JIANG W J, et al. Study on preparation of calcium propionate from waste eggshell[J]. Shandong Chemical Industry,2021,50(21):49−50, 53. doi: 10.3969/j.issn.1008-021X.2021.21.019
[4]	李建科, 孟永宏, 刘柳, 等. 我国食品工业副产物资源化利用现状[J]. 食品科学技术学报,2021,39(6):1−13. [LI J K, MENG Y H, LIU L, et al. Utilization of food industry by-products in China[J]. Journal of Food Science and Technology,2021,39(6):1−13. doi: 10.12301/j.issn.2095-6002.2021.06.001
[5]	李飞龙, 余思瑶, 肖调义, 等. 发酵豆渣的营养特性及其在水产饲料中的应用[J]. 饲料研究,2021,44(18):142−146. [LI F L, YU S Y, XIAO T Y, et al. Nutritional properties of fermented okara and its application in aquatic feed[J]. Feed Research,2021,44(18):142−146. doi: 10.13557/j.cnki.issn1002-2813.2021.18.032
[6]	姜慧燕, 邹礼根, 翁丽萍, 等. 豆渣营养成分分析及蛋白质营养价值评价[J]. 食品工业,2020,41(6):325−328. [JIANG H Y, ZOU L G, WENG L P, et al. Nutritional components analyzation and protein nutrition evaluation of soybean residue[J]. The Food Industry,2020,41(6):325−328.
[7]	SULAGNA G, LEE L J J, NING C W. Analysis of improved nutritional composition of potential functional food (okara) after probiotic solid-state fermentation[J]. Journal of Agricultural and Food Chemistry,2018,66:5373−5381. doi: 10.1021/acs.jafc.8b00971
[8]	孙万春, 周家昊, 俞巧钢, 等. 豆渣、猪粪有机肥施用水平对梨产量品质及土壤肥力的影响[J]. 果树学报,2022,39(9):1628−1638. [SUN W C, ZHOU J H, YU Q G, et al. Effects of different application rates of organic fertilizers derived from soybean residue and pig manure on yield, quality and soil fertility of pear and soil fertility[J]. Journal of Fruit Science,2022,39(9):1628−1638. doi: 10.13925/j.cnki.gsxb.20220156
[9]	李玉美, 白洁, 田旭, 等. 新型工艺豆渣在面包中的应用研究[J]. 食品科技,2018,43(10):207−211. [LI Y M, BAI J, TIAN X, et al. Application of NSF in bread[J]. Food Science and Technology,2018,43(10):207−211. doi: 10.13684/j.cnki.spkj.2018.10.035
[10]	李必为. 豆渣作为蛋白源在水产饲料中的研究进展[J]. 江西水产科技,2021(6):44−45. [LI B W. Research progress of soybean dregs as protein source in aquatic feed[J]. Jiangxi Fishery Science and Technology,2021(6):44−45. doi: 10.3969/j.issn.1006-3188.2021.06.016
[11]	李继彬. 高效低成本的豆渣生物饲料发酵转化机制的研究[D]. 镇江: 江苏大学, 2019 LI J B. Study on high-efficiency and low-cost fermentation and transformation mechanism of soybean dregs biological feed[D]. Zhenjiang: Jiangsu University, 2019.
[12]	李搏远. 利用响应面优化以鸡蛋壳为原材料制备醋酸钙、柠檬酸钙和丙酸钙的工艺研究[D]. 长春: 吉林大学, 2020 LI B Y. Optimization of the preparation of calcium acetate, calcium citrate and calcium propionate from waste eggshell by response surface methodol[D]. Changchun: Jilin University, 2020.
[13]	刘元, 姚骏, 王玥玮, 等. 蛋壳深加工产业现状[J]. 食品研究与开发,2020,41(23):220−224. [LIU Y, YAO J, WANG Y W, et al. Present situation of eggshell deep processing industry[J]. Food Research and Development,2020,41(23):220−224. doi: 10.12161/j.issn.1005-6521.2020.23.036
[14]	HE W, GAO Y A, GUO Z Q, et al. Effects of fermented wheat bran and yeast culture on growth performance, immunity and intestinal microflora in growing-finishing pigs[J]. J Anim Sci, 2021, 99(11): 1-13.
[15]	LI J T, TAO L Y, ZHANG R, et al. Effects of fermented feed on growth performance, nutrient metabolism and cecal microflora of broilers[J]. Animal Bioscience,2022,35:596−604. doi: 10.5713/ab.21.0333
[16]	张国玉, 柴娟, 李新民, 等. 番茄皮渣发酵饲料的制备及养牛试验[J]. 贵州畜牧兽医,2022,46(4):22−24. [ZHANG G Y, CHAI J, LI X M, ea al. Preparation of tomato skin residue fermented feed and cattle raising test[J]. Guizhou Journal of Animal Husbandry and Veterinary Medicine,2022,46(4):22−24. doi: 10.3969/j.issn.1007-1474.2022.04.008
[17]	童丹. 豆渣发酵方法及在肉牛饲料中应用的初步研究[D]. 呼和浩特: 内蒙古民族大学, 2020 TONG D. Study on fermentation method of soybean residue and application in beef cattle feed[D]. Hohhot: Inner Mongolia University for Nationalities, 2020.
[18]	刘长姣, 杨越越, 王妮, 等. 茚三酮比色法测定黄秋葵氨基酸含量的不确定度评定[J]. 粮食与油脂,2019,32(9):92−95. [LIU C J, YANG Y Y, WANG N, et al. Uncertainty evaluation for determining amino acids content in okra by ninhydrin colorimetric method[J]. Cereals and Oils,2019,32(9):92−95. doi: 10.3969/j.issn.1008-9578.2019.09.023
[19]	范阳, 齐伟彪, 朱崇淼, 等. 发酵豆渣工艺的优化及对营养成分的影响[J]. 畜牧与兽医,2021,53(12):17−22. [FAN Y, QI W B, ZHU C M, et al. Fermentation process of optimization of soybean residue feed and consequent nutrients changes[J]. Animal Husbandry and Veterinary Medicine,2021,53(12):17−22.
[20]	苏亮. 乳酸菌发酵蛋壳粉改善豆粕饲料的钙含量[D]. 长春: 吉林农业大学, 2018 SU L. Utilization of eggshell powder with lactic acid bacteria fermentation for improving calcium content in soybean meal feed[D]. Changchun: Jilin Agricultural University, 2018.
[21]	卢智琴, 李惠霞, 罗宁, 等. 禾谷孢囊线虫生防真菌AT9的固体发酵条件优化[J]. 草原与草坪,2020,40(1):35−40, 48. [LU Z Q, LI H X, LUO N, et al. Optimization of solid fermentation conditions of Aspergillus terreus for wheat cyst nematode biocontrol[J]. Grassland and Turf,2020,40(1):35−40, 48. doi: 10.13817/j.cnki.cyycp.2020.01.005
[22]	张琦伟. 益生菌发酵芒果原浆的研究及灭活型发酵芒果原浆饮料的研制[D]. 长春: 吉林大学, 2018 ZHANG Q W. The research on the fermented mango pulp of probiotics and the development of the inactivated fermented mango pulp beverage[D]. Changchun: Jilin University, 2018.
[23]	蒲丹丹, 李艳, 赵丽红, 等. 简青霉CEF-818固体发酵工艺优化[J]. 中国生物防治学报,2022,38(3):636−644. [PU D D, LI Y, ZHAO L H, et al. Optimization of solid-state fermentation process of Penicillium simplicissimum CEF-818[J]. Chinese Journal of Biological Control,2022,38(3):636−644. doi: 10.16409/j.cnki.2095-039x.2022.02.026
[24]	史毅, 陈文彬, 张博文, 等. 内生菌发酵工艺的研究进展[J]. 江西中医药,2018,49(9):76−80. [SHI Y, CHEN W B, ZHANG B W, et al. Research progress of endophyte fermentation technology[J]. Jiangxi Journal of Traditional Chinese Medicine,2018,49(9):76−80.
[25]	黄翔, 陶蕾, 杨燃, 等. 复合乳酸菌发酵蛋壳制备乳酸钙[J]. 食品科学,2019,40(20):159−165. [HUANG X, TAO L, YANG R, et al. Preparation of calcium lactate from eggshell by mixed-culture fermentation of lactic acid bacteria[J]. Food Science,2019,40(20):159−165. doi: 10.7506/spkx1002-6630-20190427-360
[26]	LI W, WANG T. Effect of solid-state fermentation with Bacillus subtilis lwo on the proteolysis and the antioxidative properties of chickpeas[J]. Int J Food Microbiol,2021,338:108988. doi: 10.1016/j.ijfoodmicro.2020.108988

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

Figures(9) / Tables(4)

Get Citation

PDF

XML

Article Metrics

Article views(25) PDF downloads(0)

Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology

doi: 10.13386/j.issn1002-0306.2022070321

Abstract

References

Proportional views

Catalog

Article Metrics

Proportional views

Related

Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology

doi: 10.13386/j.issn1002-0306.2022070321

Abstract

References

Proportional views

Catalog

Article Metrics

Proportional views

Related

Export File

Citation

Format

Content