Isolation and Characterization of a<i> Pueraria lobata</i> Protein and Its Self-assembled Nanoparticles Properties

LIN Dai; GAO Guanzhen; ZHOU Jianwu; KE Lijing; RAO Pingfan

doi:10.13386/j.issn1002-0306.2022100193

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May. 2023

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JOURNAL OF MECHANICAL ENGINEERING > 2023 > 44(9): 20-26.

LIN Dai, GAO Guanzhen, ZHOU Jianwu, et al. Isolation and Characterization of a Pueraria lobata Protein and Its Self-assembled Nanoparticles Properties[J]. Science and Technology of Food Industry, 2023, 44(9): 20−26. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100193

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Isolation and Characterization of a Pueraria lobata Protein and Its Self-assembled Nanoparticles Properties

doi: 10.13386/j.issn1002-0306.2022100193

LIN Dai^{1, 2
,},
GAO Guanzhen²,
ZHOU Jianwu^{2,*
,
,},
KE Lijing²,
RAO Pingfan²

1.
College of Public Health, Fujian Medical University, Fuzhou 350122, China
2.
College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China

Received Date: 19 Oct 2022
Issue Publish Date: 01 May 2023

Abstract

Abstract

Objective: To purify and characterize a water-soluble protein from Pueraria lobata and to fabricate its nanoparticles by heating-induced assembly. Methods: Pueraria lobata protein was purified by anion exchange chromatography High Q, and its molecular weight and amino acid sequence were determined by SDS-PAGE and N-terminal sequencing. The particle size, optical dispersion intensity and Zeta potential of protein nanoparticles were measured by laser-scattering particle analyzer. The drug loading efficiency of the nanocarrier was determined by chromatography. Results: A major water-soluble protein, named PP, was purified from Pueraria lobata and sequenced with a N-terminal amino acid sequence of DFVYDMCGNVLNGGTYYIL. PP was identified as a novel trypsin inhibitor by NCBI database searching and rypsin inhibitory assay. PP was also well-stabilized in the pH2~10 and 20~50 °C ranges. After heating PP solution (0.1 mg/mL, pH6.0) for 60 minutes at 100 ℃, homogenous nanoparticles (PP-NPs) were harvested. These PP-NPs had a particle size of 172.78 nm and a Zeta potential of −25.40 mV. The puerarin and berberine were effectively loaded onto PP-NPs, with loading efficiency of 33.83% and 24.61%, respectively. Conclusion: The major water-soluble Pueraria lobata protein PP can be fabricated into protein nanoparticles by heating-induced assembly, indicating a potential as drug carriers.
- Pueraria lobata,
- nanoparticles,
- self-assembly,
- trypsin inhibitor

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References

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Isolation and Characterization of a Pueraria lobata Protein and Its Self-assembled Nanoparticles Properties

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