The potential role of calnexin in the activation of cardiac fibroblasts

Geer TIAN; Mingyue ZHAO; Junteng ZHOU; Yue QUAN; Wenchao WU; Xiaojing LIU

doi:10.7507/1001-5515.202001052

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Mar 2020

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JOURNAL OF MECHANICAL ENGINEERING > 2020 > 37(3): 450-459.

Geer TIAN, Mingyue ZHAO, Junteng ZHOU, Yue QUAN, Wenchao WU, Xiaojing LIU. The potential role of calnexin in the activation of cardiac fibroblasts[J]. JOURNAL OF MECHANICAL ENGINEERING, 2020, 37(3): 450-459. doi: 10.7507/1001-5515.202001052

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Geer TIAN, Mingyue ZHAO, Junteng ZHOU, Yue QUAN, Wenchao WU, Xiaojing LIU. The potential role of calnexin in the activation of cardiac fibroblasts[J]. JOURNAL OF MECHANICAL ENGINEERING, 2020, 37(3): 450-459. doi: 10.7507/1001-5515.202001052

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The potential role of calnexin in the activation of cardiac fibroblasts

doi: 10.7507/1001-5515.202001052

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Corresponding author: LIU Xiaojing, Email: liuxq@scu.edu.cn
Received Date: 20 Jan 2020
Rev Recd Date: 17 Apr 2020
Publish Date: 17 Mar 2020

Abstract

Abstract

Calnexin is a lectin-like molecular chaperone protein on the endoplasmic reticulum, mediating unfolded protein responses, the endoplasmic reticulum Ca²⁺ homeostasis, and Ca²⁺ signals conduction. In recent years, studies have found that calnexin plays a key role in the heart diseases. This study aims to explore the role of calnexin in the activation of cardiac fibroblasts. A transverse aortic constriction (TAC) mouse model was established to observe the activation of cardiac fibroblasts in vivo, and the in vitro cardiac fibroblasts activation model was established by transforming growth factor β1 (TGFβ1) stimulation. The adenovirus was respectively used to gene overexpression and silencing calnexin in cardiac fibroblasts to elucidate the relationship between calnexin and cardiac fibroblasts activation, as well as the possible underlying mechanism. We confirmed the establishment of TAC model by echocardiography, hematoxylin-eosin, Masson, and Sirius red staining, and detecting the expression of cardiac fibrosis markers in cardiac tissues. After TGFβ1 stimulation, markers of the activation of cardiac fibroblast, and proliferation and migration of cardiac fibroblast were detected by quantitative PCR, Western blot, EdU assay, and wound healing assay respectively. The results showed that the calnexin expression was reduced in both the TAC mice model and the activated cardiac fibroblasts. The overexpression of calnexin relieved cardiac fibroblasts activation, in contrast, the silencing of calnexin promoted cardiac fibroblasts activation. Furthermore, we found that the endoplasmic reticulum stress was activated during cardiac fibroblasts activation, and endoplasmic reticulum stress was relieved after overexpression of calnexin. Conversely, after the silencing of calnexin, endoplasmic reticulum stress was further aggravated, accompanying with the activation of cardiac fibroblasts. Our data suggest that the overexpression of calnexin may prevent cardiac fibroblasts against activation by alleviating endoplasmic reticulum stress.
- calnexin,
- transverse aortic constriction,
- cardiac fibrosis,
- cardiac fibroblast activation,
- endoplasmic reticulum stress

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References

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The potential role of calnexin in the activation of cardiac fibroblasts

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