A hybrid bacterium with tumor-associated macrophage polarization for enhanced photothermal-immunotherapy

Jingya Zhao; Huabei Huang; Jinyan Zhao; Xiang Xiong; Sibo Zheng; Xiaoqing Wei; Shaobing Zhou

doi:10.1016/j.apsb.2021.10.019

A hybrid bacterium with tumor-associated macrophage polarization for enhanced photothermal-immunotherapy

doi: 10.1016/j.apsb.2021.10.019

Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China

基金项目:

This study was supported by the National Natural Science Foundation of China (51725303, 81701831, 52033007), the Sichuan Science and Technology Program (2020YFH0046, China) and the Fundamental Research Funds for the Central Universities (2682021CG017, China). The authors thank Analytical and Testing Center of Southwest Jiaotong University.

详细信息

通讯作者:
Shaobing Zhou,E-mail:shaobingzhou@swjtu.edu.cn

中图分类号: https://www.sciencedirect.com/science/article/pii/S2211383521004111/pdf?md5=1c740b2c325d0300ee1951067feeea53&pid=1-s2.0-S2211383521004111-main.pdf
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出版历程
- 收稿日期: 2021-08-08
- 修回日期: 2021-09-18
- 录用日期: 2021-10-15
- 网络出版日期: 2023-03-17

A hybrid bacterium with tumor-associated macrophage polarization for enhanced photothermal-immunotherapy

Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China

Funds:

摘要

摘要: Remodeling the tumor microenvironment through reprogramming tumor-associated macrophages (TAMs) and increasing the immunogenicity of tumors via immunogenic cell death (ICD) have been emerging as promising anticancer immunotherapy strategies. However, the heterogeneous distribution of TAMs in tumor tissues and the heterogeneity of the tumor cells make the immune activation challenging. To overcome these dilemmas, a hybrid bacterium with tumor targeting and penetration, TAM polarization, and photothermal conversion capabilities is developed for improving antitumor immunotherapy in vivo. The hybrid bacteria (B.b@QDs) are prepared by loading Ag₂S quantum dots (QDs) on the Bifidobacterium bifidum (B.b) through electrostatic interactions. The hybrid bacteria with hypoxia targeting ability can effectively accumulate and penetrate the tumor tissues, enabling the B.b to fully contact with the TAMs and mediate their polarization toward M1 phenotype to reverse the immunosuppressive tumor microenvironment. It also enables to overcome the intratumoral heterogeneity and obtain abundant tumor-associated antigens by coupling tumor penetration of the B.b with photothermal effect of the QDs, resulting in an enhanced immune effect. This strategy that combines B.b-triggered TAM polarization and QD-induced ICD achieved a remarkable inhibition of tumor growth in orthotopic breast cancer.
- Immunotherapy /
- Tumor-associated macrophage /
- Immunogenic cell death /
- Bifidobacterium /
- Quantum dot
Abstract: Remodeling the tumor microenvironment through reprogramming tumor-associated macrophages (TAMs) and increasing the immunogenicity of tumors via immunogenic cell death (ICD) have been emerging as promising anticancer immunotherapy strategies. However, the heterogeneous distribution of TAMs in tumor tissues and the heterogeneity of the tumor cells make the immune activation challenging. To overcome these dilemmas, a hybrid bacterium with tumor targeting and penetration, TAM polarization, and photothermal conversion capabilities is developed for improving antitumor immunotherapy in vivo. The hybrid bacteria (B.b@QDs) are prepared by loading Ag₂S quantum dots (QDs) on the Bifidobacterium bifidum (B.b) through electrostatic interactions. The hybrid bacteria with hypoxia targeting ability can effectively accumulate and penetrate the tumor tissues, enabling the B.b to fully contact with the TAMs and mediate their polarization toward M1 phenotype to reverse the immunosuppressive tumor microenvironment. It also enables to overcome the intratumoral heterogeneity and obtain abundant tumor-associated antigens by coupling tumor penetration of the B.b with photothermal effect of the QDs, resulting in an enhanced immune effect. This strategy that combines B.b-triggered TAM polarization and QD-induced ICD achieved a remarkable inhibition of tumor growth in orthotopic breast cancer.
- Immunotherapy /
- Tumor-associated macrophage /
- Immunogenic cell death /
- Bifidobacterium /
- Quantum dot

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参考文献(44)

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A hybrid bacterium with tumor-associated macrophage polarization for enhanced photothermal-immunotherapy

doi: 10.1016/j.apsb.2021.10.019

通讯作者:
Shaobing Zhou,E-mail:shaobingzhou@swjtu.edu.cn

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A hybrid bacterium with tumor-associated macrophage polarization for enhanced photothermal-immunotherapy

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A hybrid bacterium with tumor-associated macrophage polarization for enhanced photothermal-immunotherapy

doi: 10.1016/j.apsb.2021.10.019

通讯作者: Shaobing Zhou,E-mail:shaobingzhou@swjtu.edu.cn

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出版历程

A hybrid bacterium with tumor-associated macrophage polarization for enhanced photothermal-immunotherapy

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出版历程

目录

通讯作者:
Shaobing Zhou,E-mail:shaobingzhou@swjtu.edu.cn