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2018-2020年上海市B/Victoria系流感病毒的流行特征及基因特性分析000

赵雪 滕峥 袁芳 姜晨彦 袁政安

赵雪,滕峥,袁芳,等. 2018-2020年上海市B/Victoria系流感病毒的流行特征及基因特性分析000[J]. 疾病监测,2023,1(1):1 − 8
引用本文: 赵雪,滕峥,袁芳,等. 2018-2020年上海市B/Victoria系流感病毒的流行特征及基因特性分析000[J]. 疾病监测,2023,1(1):1 − 8
Zhao X.,Teng Z.,Yuan F.,et al. Antigenic and genetic characteristics of influenza B virus/Victoria lineage in Shanghai, 2018–2020[J]. Dis Surveill, 2023, 1(1): 1 − 8
Citation: Zhao X.,Teng Z.,Yuan F.,et al. Antigenic and genetic characteristics of influenza B virus/Victoria lineage in Shanghai, 2018–2020[J]. Dis Surveill, 2023, 1(1): 1 − 8

2018-2020年上海市B/Victoria系流感病毒的流行特征及基因特性分析000

基金项目: 上海市卫生健康委青年项目(No. 2016Y0107);国家科技重大专项(No. 2017ZX10103009–003)
详细信息
    作者简介:

    赵雪,女,河北省衡水市人,博士,主要从事流感等呼吸道病毒的监测及研究工作

    滕峥,女,上海市人,本科,主任技师,主要从事呼吸道肠道等传染病监测及研究工作

  • 中图分类号: R211;R373

Antigenic and genetic characteristics of influenza B virus/Victoria lineage in Shanghai, 2018–2020

Funds: This study was supported by the Youth Project of Shanghai Health Commission (No. 2016Y0107) and National Science and Technology Major Project (No. 2017ZX10103009–003)
  • 摘要:
    目的  分析2018 — 2020年上海市分离的B/Victoria系流感毒株与疫苗株的匹配性及基因变异情况。
    方法  利用血凝抑制实验,对142株B/Victoria系流感毒株进行了抗原性分析,并选取了63株开展了血凝素(HA)及神经氨酸酶(NA)基因序列分析。
    结果  2018 — 2020年上海市流行的B/Victoria系流感毒株主要属于V1A.3分支,少数属于V1A.1分支及未缺失的V1A分支;抗原性分析显示,26.76%的流行株是疫苗株B/Colorado/06/2017鸡胚株的低反应株,与疫苗株相比,V1A.3分支流行株在HA蛋白的120环、150环、160环及190螺旋等关键的抗原决定簇及受体结合关键部位发生了5个氨基酸位点改变。
    结论  2018 — 2020监测年度B/Victoria系流行株与世界卫生组织推荐的疫苗株组分B/Colorado/06/2017匹配性不佳,仍需密切监测流行株的谱系及变异情况,为疫苗株的筛选提供可靠的数据。

     

  • 图  2018-2020年上海市B/Victoria系流感病毒real-time RCR鉴定阳性病例

    Figure  1.  Cases of influenza B virus/Victoria lineage infection identified by real time PCR in Shanghai, 2018–2020

    图  2018-2020年上海市B/Victoria系流感病毒感染人群年龄分布

    Figure  2.  Age distribution of people infected with influenza B virus/Victoria lineage in Shanghai, 2018–2020

    图  2018-2020年监测年度上海市分离的B/Victoria系流感病毒HA基因进化树

    注: . 2009—2012、2013—2018年度疫苗株;●. 2018—2020年度疫苗株;▲. 2020—2021年度疫苗株

    Figure  3.  Phylogenetic tree of HA genes of influenza B virus/Victoria lineage isolated in Shanghai, 2018–2020

    图  2018-2020年监测年度上海市分离的B/Victoria系流感病毒NA基因进化树

    注: . 2009—2012、2013—2018年度疫苗株;●. 2018—2020年度疫苗株;▲. 2020—2021年度疫苗株

    Figure  4.  Phylogenetic tree of NA genes of influenza B virus/Victoria lineage isolated in Shanghai, 2018–2020

    表  2  2018-2020年监测年度上海市分离的B/Victoria系流感病毒HA蛋白位点变异情况

    Table  2.   Mutation sites of amino acid of HA genes of influenza B virus/Victoria lineage isolated in Shanghai, 2018–2020

    分支 位点 B/Colorado/06/2017 氨基酸变异情况 是否属于抗原决定簇 是否属于受体结合部位 是否属于糖基化位点
    V1A.3 133 G G133R 120环 
    V1A.3  136 K  K136E 120环  140环 
    V1A.3 164 D  缺失 160环 
    V1A.3 180 V  V180I
    V1A.3 197 T  T197N/T197S 190螺旋 190螺旋 增加NETQ
    V1A.3 498 K K498R
    V1A.3 184 G  G184E
    V1A.3 252 V  V252M
    V1A.3 279 R  R279K
    V1A 129 G  G129D 120环 
    V1A 162 K  不缺失 160环 
    V1A 163 N  不缺失 160环 
    V1A 180 V  V180I
    V1A 197 T  T197N/T197S 190螺旋 190螺旋 增加NETQ
    V1A 498 K  K498R
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-08-27
  • 录用日期:  2022-10-09
  • 网络出版日期:  2022-11-02

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