基于光谱法的特级初榨橄榄油快速鉴伪技术

唐聪; 邵士俊; 温玉洁; 梁卿; 董树清

doi:10.13386/j.issn1002-0306.2022060131

基于光谱法的特级初榨橄榄油快速鉴伪技术

doi: 10.13386/j.issn1002-0306.2022060131

唐聪^{1, 2,},
邵士俊^2,*, ,,
温玉洁^{1, 2},
梁卿²,
董树清^2,*, ,

1.
中国科学院兰州化学物理研究所，中国科学院西北植物资源化学重点实验室，甘肃省天然药物重点实验室，甘肃兰州 730000
2.
中国科学院大学，北京 100049

基金项目: 国家重点研发计划（No.2019YFD1002403）；甘肃省重点研发计划（No.20YF8FA003）。

详细信息

作者简介:
唐聪（1996−），男，博士研究生，研究方向：油脂鉴别，E-mail：Tangcong@licp.cas.cn

通讯作者:
邵士俊（1968−），男，博士，研究员，研究方向：分子识别与分离分析方法研究，E-mail：sjshao@licp.cas.cn

董树清（1978−），女，博士，副研究员，研究方向：生物分析、分离分析，E-mail：sqdong@licp.cas.cn

中图分类号: TS227
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出版历程
- 收稿日期: 2022-06-15
- 刊出日期: 2023-05-01

Rapid Identification of Extra Virgin Olive Oil by Spectrometry

TANG Cong^{1, 2
,},
SHAO Shijun^{2,*
, ,},
WEN Yujie^{1, 2},
LIANG Qing²,
DONG Shuqing^{2,*
, ,}

1.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China

摘要

摘要: 本文研究了特级初榨橄榄油中掺入不同比例橄榄果榨油（精炼橄榄油）、菜籽油、玉米油和大豆油的光谱特征，采用荧光光谱和紫外光谱，对掺假样品及纯油样品进行了快速检测。结果表明，特级初榨橄榄油的光谱特征与其他植物油之间差异较大，且掺假体积与吸光度之间存在良好的线性关系（R²>0.89），实现了特级初榨橄榄油的定性鉴别与定量检测，建立了特级初榨橄榄油质量控制体系及其掺假检测分析技术，最低检出限为1%，线性范围为5%~100%（v/v）。系统聚类分析将所有特级初榨橄榄油准确地分为一个亚类，也佐证了此方法的稳定性与可靠性。这种简单快捷的检测技术，有助于特级初榨橄榄油实时、在线橄榄油检测分析技术的研发，为我国橄榄油品质鉴定及产业发展提供有利的技术保障。
- 特级初榨橄榄油 /
- 荧光光谱 /
- 吸收光谱 /
- 鉴伪
Abstract: In this paper, the spectral characteristics of extra virgin olive oil mixed with different proportions of olive oil (refined olive oil), rapeseed oil, corn oil and soybean oil were investigated. The adulterated samples and pure oil samples were rapidly detected by fluorescence spectroscopy and ultraviolet spectroscopy. The results showed that the spectral characteristics of extra virgin olive oil were quite different from other vegetable oils, and there was a good linear relationship between the adulteration volume and absorbance of adulterated extra virgin olive oil (R²>0.89), which realized the qualitative identification and quantitative detection of extra virgin olive oil. The quality control system of extra virgin olive oil and its adulteration detection and analysis technology was established. The minimum detection limit was 1% and the linear range was 5%~100% (v/v). Systematic cluster analysis accurately classified all extra virgin olive oils into one subclass, which also confirmed the stability and reliability of this method. This simple and fast detection technology is conducive to the research and development of real-time, online olive oil detection and analysis technology for extra virgin olive oil portable detection equipment, and provides a favorable technical guarantee for Chinese olive oil quality identification and industrial development.
- extra virgin olive oil /
- fluorescence /
- absorption spectrum /
- authentication

HTML全文

图 1 各类植物油的三维荧光光谱图

注：a，a^*：特级初榨橄榄油；b，b^*：橄榄果渣油；c，c^*：菜籽油；d，d^*：玉米油；e，e^*：大豆油。

Figure 1. 3D fluorescence spectrogram of various vegetable oils

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图 2 掺入不同体积百分比菜籽油的特级初榨橄榄油的三维荧光光谱图

注：a，a^* ：0%；b，b^* ：20%；c，c^* ：40%；d，d^* ：80%；e，e^* ：100%。

Figure 2. 3D fluorescence spectra of extra virgin olive oil incorporated with different volume percentages of rapeseed oil

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图 3 各类植物油的发射荧光光谱（a）和可见吸收光谱（b）

Figure 3. Emission fluorescence spectrum (a) and UV absorption spectrum (b) of various vegetable oils

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图 4 掺入不同种类植物油的体积百分数与特级初榨橄榄油（EVOO-1）吸光度的关系

注：a：RVO-1；b：RVO-3；c：RO；d：CO；e：SO。

Figure 4. Relationship between incorporation amount and absorbance of EVOO-1 ked with different oils

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图 5 掺入油品不同的特级初榨橄榄油的吸光度与掺入体积百分比的标准曲线

注：a：EVOO-1；b：EVOO-5；c：EVOO-6；d：EVOO-7。

Figure 5. Standard curve of absorbance versus volume percent of blended extra virgin olive oil with different blends

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图 6 各类植物油的系统聚类分析

Figure 6. Systematic cluster analysis of various vegetable oils

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表 1 样品信息

Table 1. Sample information

类别	序号	产地	品牌	品种	加工工艺	质量等级
特级初榨橄榄油	EVOO-1	甘肃	田园	鄂植8号	冷榨	特级初榨
	EVOO-2	甘肃	田园	Leccino	冷榨	特级初榨
	EVOO-3	甘肃	祥宇	−	冷榨	特级初榨
	EVOO-4	山东	鲁花	−	冷榨	特级初榨
	EVOO-5	湖北	鑫榄园	−	冷榨	特级初榨
	EVOO-6	意大利	欧丽薇兰	−	冷榨	特级初榨
	EVOO-7	西班牙	贝蒂斯	Hojiblanca，Picual	冷榨	特级初榨
橄榄果渣油	RVO-1	意大利	欧丽薇兰	−	混合型	精炼一级
	RVO-2	西班牙	西奥图	−	混合型	精炼一级
	RVO-3	江西	念食安	−	混合型	精炼一级
菜籽油	RO	中国	金龙鱼	−	精炼	精炼一级
玉米油	CO	中国	长寿花	−	精炼	精炼一级
大豆油	SO	中国	金龙鱼	−	精炼	精炼一级
注：“−”表示该产品中未注明榨油的油橄榄品种。

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表 2 线性拟合度（R²）和精密度（RSD）

Table 2. Linear fit (R²) and precision (RSD)

	EVOO-1		EVOO-5		EVOO-6		EVOO-7
	R²	RSD（%）	R²	RSD（%）	R²	RSD（%）	R²	RSD（%）
RVO-1	0.9928	0.10	0.9278	1.10	0.9838	0.40	0.9268	3.42
RVO-3	0.9957	0.12	0.9119	0.35	0.9864	0.97	0.9517	0.31
RO	0.9950	0.19	0.9264	0.51	0.9485	0.10	0.9584	0.98
CO	0.9872	0.34	0.8942	0.21	0.9714	0.03	0.9793	0.63
SO	0.9775	1.80	0.8929	0.53	0.9633	0.61	0.9439	0.88

下载: 导出CSV

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