PEG修饰EGCG/Cur复合脂质体的制备与抗氧化研究

李亮; 袁传勋; 王敏; 朱梦男; 金日生

doi:10.13386/j.issn1002-0306.2022060218

PEG修饰EGCG/Cur复合脂质体的制备与抗氧化研究

doi: 10.13386/j.issn1002-0306.2022060218

李亮^{1, 2,},
袁传勋^{1, 2},
王敏^{1, 2},
朱梦男^{1, 2},
金日生^{1, 2,*, ,}

1.
合肥工业大学食品与生物工程学院，安徽合肥 230601
2.
合肥工业大学农产品生物化工教育部工程中心，安徽合肥 230601

基金项目: 安徽省科技重大专项（202003a06020011）。

详细信息

作者简介:
李亮（1996−），男，硕士研究生，研究方向：小分子活性物质包埋，E-mail：55884910@qq.com

通讯作者:
金日生（1982−），男，博士，助理研究员，研究方向：天然活性物质研究与开发，E-mail：jinrisheng@hfut.edu.cn

中图分类号: TS201.2
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- 文章访问数: 23
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- 被引次数: 0
出版历程
- 收稿日期: 2022-06-24
- 刊出日期: 2023-05-01

Preparation and Antioxidant Study of PEG-Modified EGCG/Cur Composite Liposomes

LI Liang^{1, 2
,},
YUAN Chuanxun^{1, 2},
WANG Min^{1, 2},
ZHU Mengnan^{1, 2},
JIN Risheng^{1, 2,*
, ,}

1.
School of Food and Bioengineering, Hefei University of Technology, Hefei 230601, China
2.
Engineering Center of Agricultural Products Biochemical Engineering, Ministry of Education, Hefei 230601, China

摘要

摘要: 本研究采用薄膜水合法制备表没食子儿茶素没食子酸酯（EGCG）/姜黄素（Cur）复合脂质体（EGCG/Cur-L），通过单因素实验确定最佳制备工艺。为了增加脂质体的稳定性，对EGCG/Cur-L表面进行二硬脂酰基磷脂酰乙醇胺-聚乙二醇2000（DSPE-PEG₂₀₀₀）修饰，并对两种脂质体的包封率、粒径分布、微观形态和修饰效果进行研究，同时采用DPPH法评价脂质体的氧化稳定性。结果表明：EGCG/Cur-L的最佳工艺为：卵磷脂与胆固醇质量比为6:1，PBS缓冲溶液pH为6.5，EGCG添加量为6 mg，Cur添加量为3 mg，水化温度为55 ℃。EGCG包封率为68.78%，Cur包封率为90.23%，平均粒径为183.8±5.4 nm，多分散系数（PDI）为0.178±0.01，平均Zeta-电位为−34.7±0.62 mV。修饰后EGCG包封率为60.31%，Cur包封率为88.53%。表面修饰后Cur的包封率无明显变化，EGCG包封率有所下降；动态光散射（DLS）和透射电子显微镜（TEM）结果表明，修饰后脂质体的平均粒径从未修饰的183.8 nm增大到374.5 nm；Zeta-电位和傅里叶红外分析结果表明DSPE-PEG₂₀₀₀成功修饰在脂质体表面，且不改变脂质体内部结构。修饰脂质体DPPH清除率最高，在15 d内氧化稳定性均大于其他脂质体，表明DSPE-PEG₂₀₀₀可以增强脂质体的抗氧化能力。
- 脂质体 /
- 表没食子儿茶素没食子酸酯 /
- 姜黄素 /
- 表面修饰 /
- 聚乙二醇
Abstract: In this study, epigallocatechin gallate (EGCG)/curcumin (Cur) composite liposomes (EGCG/Cur-L) were prepared by thin-film hydrophoresis, and the optimal preparation process was determined by single-factor test. To increase the stability of liposomes, the surface of the composite liposomes was modified with distearoyl phosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG₂₀₀₀), and the encapsulation rate, particle size distribution, micromorphology, and modification effect of both liposomes were investigated, and the oxidative stability of liposomes was evaluated by DPPH method. The results showed that the optimal process for the composite liposomes was: Lecithin to cholesterol mass ratio of 6:1, PBS buffer solution pH of 6.5, EGCG added amount 6 mg, Cur added amount 3 mg, and hydration temperature of 55 ℃. The encapsulation rate of EGCG was 68.78%, the encapsulation rate of Cur was 90.23%, the average particle size was 183.8±5.4 nm, the polydispersity coefficient (PDI) was 0.178±0.01, and the average Zeta-potential was −34.7±0.62 mV. The encapsulation rate of EGCG after modification was 60.31%, and the encapsulation rate of Cur was 88.53%. After surface modification, the encapsulation rate of Cur did not change significantly, and the encapsulation rate of EGCG decreased, dynamic light scattering (DLS) and transmission electron microscopy (TEM) results showed that the average particle size of the modified liposomes increased from the unmodified 183.8 nm to 374.5 nm, Zeta-potential and Fourier infrared analysis indicated that DSPE-PEG₂₀₀₀ was successfully modified on the liposome surface and did not change the internal structure of the liposomes. The modified liposomes had the highest DPPH scavenging rate and all had greater oxidative stability than other liposomes within 15 days, indicating that DSPE-PEG₂₀₀₀ could enhance the antioxidant capacity of liposomes.
- liposomes /
- epigallocatechin gallate /
- curcumin /
- surface modification /
- polyethylene glycol

HTML全文

图 1 卵磷脂与胆固醇质量比对EGCG/Cur-L包封率及粒径分布的影响

Figure 1. Effect of mass ratio of lecithin to cholesterol on EE of EGCG/Cur-L and particle size distribution

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图 2 Cur添加量对EGCG/Cur-L包封率及粒径分布的影响

Figure 2. Effect of concentrations of Cur on EE of EGCG/Cur-L and particle size distribution

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图 3 水化温度对EGCG/Cur-L包封率及粒径分布的影响

Figure 3. Effect of temperature of EGCG/Cur on EE of EGCG/Cur-L and particle size distribution

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图 4 PBS缓冲液的pH对EGCG/Cur-L包封率及粒径分布的影响

Figure 4. Effect of pH of PBS buffer on EE of EGCG/Cur-L and particle size distribution

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图 5 un-L、EGCG/Cur-L、DSPE-PEG₂₀₀₀-L的粒度分布

Figure 5. Particle size distribution of un-L、EGCG/Cur-L、DSPE-PEG₂₀₀₀-L

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图 6 EGCG/Cur-L、DSPE-PEG₂₀₀₀-L的透射电镜图

Figure 6. TEM images of EGCG/Cur-L and DSPE-PEG₂₀₀₀-L

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图 7 Cur、EGCG、DSPE-PEG₂₀₀₀、un-L、EGCG/Cur-L和DSPE-PEG₂₀₀₀-L的红外光谱图

Figure 7. Infrared spectra of Cur, EGCG, DSPE-PEG₂₀₀₀, un-L, EGCG/Cur-L and DSPE-PEG₂₀₀₀-L

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图 8 un-L、Cur-L、EGCG-L、EGCG/Cur-L、DSPE-PEG₂₀₀₀-L的DPPH自由基清除能力

Figure 8. DPPH radical scavening activity of un-L, Cur-L, EGCG-L, EGCG/Cur-L and DSPE-PEG₂₀₀₀-L

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图 9 un-L、Cur-L、EGCG-L、EGCG/Cur-L、DSPE-PEG₂₀₀₀-L的DPPH自由基清除能力稳定性

Figure 9. Stability of DPPH radical scavenging ability of un-L, Cur-L, EGCG-L, EGCG/Cur-L and DSPE-PEG₂₀₀₀-L

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