负载花色苷的壳聚糖-水杨醛水凝胶的制备及性能

李煦; 董翠芳; 刘长霞; 凌含悦; 李英; 范小振

doi:10.13386/j.issn1002-0306.2022060293

负载花色苷的壳聚糖-水杨醛水凝胶的制备及性能

doi: 10.13386/j.issn1002-0306.2022060293

沧州师范学院化学与化工系，河北沧州 061000

基金项目: 沧州市重点研发项目（213104001）；河北省高等学校科学研究项目（ZC2022044）；河北省“三三三人才工程”资助项目（A202001102）；河北省高等教育教学改革研究与实践项目（2020GJJG368）；沧州师范学院科研创新团队资助（cxtdl1901）。

详细信息

作者简介:
李煦（1984−），女，硕士，副教授，研究方向：天然产物化学，E-mail：lixu__2004@163.com

通讯作者:
范小振（1966−），男，硕士，教授，研究方向：天然产物化学，E-mail：fxz0315@126.com

中图分类号: TQ317.4
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- 文章访问数: 21
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- 被引次数: 0
出版历程
- 收稿日期: 2022-06-29
- 刊出日期: 2023-05-01

Preparation and Properties of Chitosan Salicylaldehyde Hydrogel Loaded with Anthocyanins

Department of Chemistry and Chemical Engineering, Cangzhou Normal University, Cangzhou 061000, China

摘要

摘要: 在蓝莓花色苷（ACNs）存在下，以水杨醛为交联剂原位构筑了负载ACNs的壳聚糖水凝胶（ACNs/CS-SA），表征了其结构和形貌，研究了其稳定性、溶胀性能和缓释性能。FT-IR和XRD表征结果表明ACNs通过物理包埋均匀分散在水凝胶三维网络结构中；TG-DTG表征结果表明凝胶包埋显著提高了ACNs的热稳定性；ACNs/CS-SA的溶胀性能和缓释性能均展现出pH响应性；在pH2.7、4.6、6.7介质中，ACNs/CS-SA 24 h累计释药率分别为74.28%±4.58%、40.72%±4.04%和15.70%±1.71%；释放过程符合Weibull方程，R²分别为0.99405、0.95165和0.99712。鉴于ACNs/CS-SA的pH响应性能和对ACNs热稳定性的提高，本研究有望为新型药物包封材料的开发和ACNs的应用提供理论和实验基础。
- 花色苷 /
- 壳聚糖水凝胶 /
- 稳定性 /
- pH响应 /
- 缓释性能 /
- 动力学
Abstract: In the presence of blueberry anthocyanins (ACNs), chitosan hydrogel crosslinking with salicylaldehyde (ACNs/CS-SA) was constructed. The structure and morphology of ACNs/CS-SA were characterized. The stability, swelling properties and ACNs controlled-release behaviors for the ACNs/CS-SA were investigated in the media of different pH. ACNs were uniformly dispersed in three-dimensional network structure of CS-SA hydrogel by physical embedding, which was confirmed by FT-IR and XRD characterization. The thermal stability of ACNs was significantly improved due to gelation, which was confirmed by TG-DTG characterization. Both the swelling properties and ACNs controlled-release behaviors for the ACNs/CS-SA exhibited pH response. In buffer media of pH2.7, 4.6 and 6.7, the cumulative release rates of ACNs for 24 h were 74.28%±4.58%, 40.72%±4.04% and 15.70%±1.71%, and the release process followed Weibull equation with the correlation coefficients R² 0.99405, 0.95165 and 0.99712, respectively. This study lays a theoretical and experimental foundation for the development of novel drug encapsulation materials and the application of ACNs owing to the ACNs/CS-SA pH responsiveness and ACNs thermal stability improvement.
- anthocyanins /
- chitosan hydrogel /
- stability /
- pH response /
- sustained release properties /
- dynamics

HTML全文

图 1 不同负载量的ACNs/CS-SA照片

Figure 1. Photos of ACNs/CS-SA with different loads

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图 2 ACNs、CS-SA和不同负载量ACNs/CS-SA的FT-IR表征

注：a：ACNs；b：CS-SA；c：20% ACNs/CS-SA；d：100% ACNs/CS-SA；e：150% ACNs/CS-SA；图3、图4同。

Figure 2. FT-IR spectra of ACNs, CS-SA and ACNs/CS-SA with different loads

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图 3 ACNs、CS-SA和不同负载量ACNs/CS-SA的UV-Vis表征

Figure 3. UV-Vis spectra of ACNs, CS-SA and ACNs/CS-SA with different loads

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图 4 ACNs、CS-SA和不同负载量ACNs/CS-SA的XRD表征

Figure 4. X-ray diffraction patterns of ACNs, CS-SA and ACNs/CS-SA with different loads

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图 5 ACNs/CS-SA组装示意图

Figure 5. Assembly mechanism of ACNs/CS-SA

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图 6 CS-SA和不同负载量ACNs/CS-SA的SEM表征

Figure 6. Assembly mechanism of CS-SA and ACNs/CS-SA with different loads

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图 7 ACNs、CS-SA和100% ACNs/CS-SA的TG（A）、DTG（B）表征

Figure 7. TG (A), DTG (B) curves of ACNs, CS-SA and 100% ACNs/CS-SA

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图 8 不同pH条件下100% ACNs/CS-SA（A）和CS-SA（B）的溶胀性能

Figure 8. Swelling performance of 100% ACNs/CS-SA (A) and CS-SA (B) with different pH

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图 9 不同pH条件下100% ACNs/CS-SA的缓释性能

Figure 9. Sustained release performance of 100% ACNs/CS-SA with different pH

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表 1 100% ACNs/CS-SA释放动力学模型分析

Table 1. Release dynamics model analysis of 100% ACNs/CS-SA

	模型	方程	R²
pH2.7	准一级	$\rm Q = 64.22519 \times [1 - \exp ( - 0.00683t)] $	0.89566
	准二级	$\rm Q = 1/(0.01328+1.63956/x) $	0.95617
	Higuchi	$\rm Q = 1.94371{t^{1/2}}+12.00928 $	0.91015
	Weibull	$\rm Q = 77.49016 \times \{ 1 - {e^{ - {{[0.00362(t+15.10121)]}^{0.7096}}}}\} $	0.99405
	Pepaas	$\rm Q = 6.90565{t^{0.33862}} $	0.95904
pH4.6	准一级	$\rm Q = 34.54011 \times [1 - \exp ( - 0.01705t)] $	0.81687
	准二级	$\rm Q = 1/(0.02534+1.21956/x) $	0.91344
	Higuchi	$\rm Q = 0.94346{t^{1/2}}+13.24074 $	0.76389
	Weibull	$\rm Q = 46.15881 \times \{ 1 - {e^{ - {{[0.00613(t - 7.72996)]}^{0.40212}}}}\} $	0.95165
	Pepaas	$\rm Q = 7.594{t^{0.24816}} $	0.88208
pH6.7	准一级	$\rm Q = 16.46357 \times [1 - \exp ( - 0.00273t)] $	0.96812
	准二级	$\rm Q = 1/(0.0473+19.09845/x) $	0.94464
	Higuchi	$\rm Q = 0.46828{t^{1/2}}+0.02852 $	0.83522
	Weibull	$\rm Q = 15.6828 \times \{ 1 - {e^{ - {{[0.00289(t+29.80457)]}^{1.63029}}}}\} $	0.99712
	Pepaas	$\rm Q = 0.61095{t^{0.45867}} $	0.84236

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