基于气相离子迁移谱技术的芋头产地鉴别方法

胡航伟; 巩敏; 梁辰; 刘凌霄; 王亮; 刘云国

doi:10.13386/j.issn1002-0306.2022070176

基于气相离子迁移谱技术的芋头产地鉴别方法

doi: 10.13386/j.issn1002-0306.2022070176

胡航伟^{1, 2,},
巩敏¹,
梁辰^{1, 2},
刘凌霄³,
王亮²,
刘云国^1,*, ,

1.
临沂大学生命科学学院，山东临沂 276000
2.
新疆大学生命科学与技术学院，新疆乌鲁木齐 830002
3.
临沂市农业科学院，山东临沂 276000

基金项目: 山东省高等学校青创人才引育计划创新团队项目（2021QCYY007）；山东省重点研发计划（医用食品专项计划）（2019YYSP026）。

详细信息

作者简介:
胡航伟（1997−），男，硕士研究生，研究方向：果蔬加工及贮藏，E-mail：hhwwwf1314@163.com

通讯作者:
刘云国（1977−），男，博士，教授，研究方向：农产品精深加工及贮藏保鲜，E-mail：yguoliu@163.com

中图分类号: TS255.1
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出版历程
- 收稿日期: 2022-07-19
- 刊出日期: 2023-05-15

Identification Method of Taro (Colocasia esculenta L.) Origin Based on Gas Chromatography-Ion Mobility Spectrometry Technology

HU Hangwei^{1, 2
,},
GONG Min¹,
LIANG Chen^{1, 2},
LIU Lingxiao³,
WANG Liang²,
LIU Yunguo^{1,*
, ,}

1.
College of Life Sciences, Linyi University, Linyi 276000, China
2.
College of Life Science and Technology, Xinjiang University, Urumqi 830002, China
3.
Linyi Academy of Agricultural Sciences, Linyi 276000, China

摘要

摘要: 分析不同产地芋头挥发性有机化合物组成差异，并构建芋头产地溯源的可视化指纹图谱。采用气相离子迁移谱法对不同产地芋头的挥发性物质进行测定，结合主成分分析（PCA）实现样品产地的快速区分，进一步筛选芋头中差异挥发性物质。结果表明，在不同产地芋头中，共检测到45个信号峰，鉴定出26种化合物，包括单体和部分化合物的二聚体。主成分分析将芋头分为3类，其中，靖江香沙芋和奉化芋头的挥发性物质较为相似，而与荔浦芋头、沙沟芋头差别很大。异丙醇、2-甲基-乙酸丁酯、辛酸甲酯是区别不同芋头的特征标记物。该方法直观、快速，为地方特色芋头的区分提供了新方法和技术支持。
- 芋头 /
- 气相离子迁移谱 /
- 化学计量学 /
- 特征标记物
Abstract: To analyze the composition of volatile organic compounds in taro from different origins, and construct visual fingerprint of taro origins. The volatile components of taro from different origins were determined by gas chromatography-ion mobility spectrometry (GC-IMS), and principal component analysis (PCA) could quickly distinguish the differences of volatile components, further screened the different volatile compounds in taro samples. Results showed that, a total of 45 signal peaks were detected in taro from different origins, and 26 compounds were identified, including monomers and dimers of some compounds. Taro samples could be divided into three categories by PCA. Among them, the volatile substances of Jingjiang taro and Fenghua taro were similar, but they were significant different from Lipu taro and Shagou taro. Isopropanol, 2-methyl-butyl acetate and methyl octanoate were regarded as characteristic markers to distinguish different taro samples. This method was intuitive and rapid, and would provide a novel method and technical support for distinguishing local characteristic taro.
- taro (Colocasia esculenta L.) /
- gas chromatography-ion mobility spectrometry /
- chemometrics /
- characteristic marker

HTML全文

图 1 基于HS-GC-IMS技术不同产地芋头样品的挥发性化合物

注：A：三维谱图；B：二维俯视谱图；C：差异谱图。

Figure 1. Volatile compounds of taro samples in different origins based on HS-GC-IMS technology

下载: 全尺寸图片幻灯片

图 2 不同地理来源的芋头样品指纹图谱

注：指纹线代表每个芋头样品中的挥发性有机化合物（VOCs），柱代表不同产地芋头样品中的VOC含量。

Figure 2. Characteristic fingerprint for taro samples from different geographical origins

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图 3 不同产地芋头挥发性物质主成分分析

Figure 3. Principal component analysis of volatile substances in taro from different origins

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图 4 不同产地芋头挥发性物质正交偏最小二乘判别分析

Figure 4. Orthogonal partial least squares-discriminant analyses of volatile substances in taro from different origins

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图 5 不同产地芋头差异挥发性物质的载荷图

Figure 5. Loadings plot of differential volatile substances of taro samples from different origins

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表 1 芋头产地基本信息

Table 1. Basic information of taro origins

编号	取样地点	样品名称
T1-1	临沂市罗庄区白沙沟村	沙沟芋头
T1-2	临沂市罗庄区唐沙沟村
T1-3	临沂市罗庄区郑旺村
T2-1	靖江市斜桥镇	靖江香沙芋
T2-2	靖江市孤山镇
T2-3	靖江市西来镇
T3-1	宁波市奉化区溪口镇	奉化芋头
T3-2	宁波市奉化区尚田镇
T3-3	宁波市奉化区萧王庙镇
T4-1	荔浦市青山镇	荔浦芋头
T4-2	荔浦市修仁镇
T4-3	荔浦市新坪镇

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表 2 HS-GC-IMS技术鉴定芋头样品中挥发性化合物

Table 2. Volatile compounds identified in taro samples by HS-GC-IMS technology

序号	中文名称	英文名称	CAS#	分子式	M_W	RI	RT	DT	备注
1	辛酸甲酯	Methyl octanoate	C111-11-5	C₉H₁₈O₂	158.2	1140.5	553.003	1.429
2	己酸甲酯	Methyl hexanoate	C106-70-7	C₇H₁₄O₂	130.2	924	284.075	1.284	M
3	己酸甲酯	Methyl hexanoate	C106-70-7	C₇H₁₄O₂	130.2	923.4	283.565	1.682	D
4	2-甲基-丁酸乙酯	ethyl 2-methylbutyrate	C7452-79-1	C₇H₁₄O₂	130.2	843.4	230.764	1.229
5	2-甲基-丁酸甲酯	methyl 2-methylbutanoate	C868-57-5	C₆H₁₂O₂	116.2	771.7	193.990	1.186	M
6	2-甲基-丁酸甲酯	methyl 2-methylbutanoate	C868-57-5	C₆H₁₂O₂	116.2	768.3	192.608	1.538	D
7	丙酸异丙酯	iso-Propyl propanoate	C637-78-5	C₆H₁₂O₂	116.2	749.9	185.142	1.185
8	丁酸甲酯	Butanoic acid methyl ester	C623-42-7	C₅H₁₀O₂	102.1	717.2	171.871	1.432
9	异丁酸甲酯	methyl 2-methylpropanoate	C547-63-7	C₅H₁₀O₂	102.1	681.3	158.323	1.440
10	2-甲基-乙酸丁酯	Acetic acid, 2-methylbutyl ester	C624-41-9	C₇H₁₄O₂	130.2	880.5	250.671	1.311	M
11	2-甲基-乙酸丁酯	Acetic acid, 2-methylbutyl ester	C624-41-9	C₇H₁₄O₂	130.2	879.3	250.031	1.706	D
12	丁酸乙酯	Ethyl butyrate	C105-54-4	C₆H₁₂O₂	116.2	791.7	203.056	1.208
13	乙酸甲酯	Methyl acetate	C79-20-9	C₃H₆O₂	74.1	522.7	115.562	1.035
14	甲酸乙酯	Ethyl formate	C109-94-4	C₃H₆O₂	74.1	503.8	110.455	1.068
15	壬醛	Nonanal	C124-19-6	C₉H₁₈O	142.2	1108.9	507.586	1.475
16	庚醛	Heptanal	C111-71-7	C₇H₁₄O	114.2	902.5	265.704	1.326
17	己醛	Hexanal	C66-25-1	C₆H₁₂O	100.2	793.8	204.220	1.254
18	2-甲基丁醛	2-methylbutanal	C96-17-3	C₅H₁₀O	86.1	661.2	152.909	1.159
19	3-甲基丁醛	3-methylbutanal	C590-86-3	C₅H₁₀O	86.1	651.4	150.270	1.170
20	α-松油烯	terpinolene	C586-62-9	C₁₀H₁₆	136.2	1083.3	470.810	1.223
21	柠檬烯	Limonene	C138-86-3	C₁₀H₁₆	136.2	1023.5	384.704	1.223
22	β-吡喃烯	1,2,6,6-tetramethylcyclohexa-1,3-diene	C514-96-5	C₁₀H₁₆	136.2	993.6	343.427	1.222
23	（Z）-罗勒烯	Cis-Ocimene	C3338-55-4	C₁₀H₁₆	136.2	1034.7	400.884	1.221
24	（E）-罗勒烯	（E）-Ocimene	C3779-61-1	C₁₀H₁₆	136.2	1044.4	414.754	1.216
25	2-甲基-1-丁醇	2-methylbutan-1-ol	C137-32-6	C₅H₁₂O	88.1	734.9	179.059	1.227
26	3-甲基-1-丁醇	3-methylbutan-1-ol	C123-51-3	C₅H₁₂O	88.1	730.1	177.124	1.241
27	异丙醇	Isopropyl alcohol	C67-63-0	C₃H₈O	60.1	505	110.775	1.092	M
28	异丙醇	Isopropyl alcohol	C67-63-0	C₃H₈O	60.1	506.2	111.094	1.217	D
29	2-庚酮	2-Heptanone	C110-43-0	C₇H₁₄O	114.2	897.2	261.183	1.263
30	2-己酮	2-Hexanone	C591-78-6	C₆H₁₂O	100.2	796.9	205.854	1.185
31	未知物1	unidentified	−	−	−	1024.8	386.645	1.356
32	未知物2	unidentified	−	−	−	1066.4	446.399	1.287
33	未知物3	unidentified	−	−	−	779.2	197.031	1.090
34	未知物4	unidentified	−	−	−	695.5	163.098	1.102
35	未知物5	unidentified	−	−	−	739	180.721	1.519
36	未知物6	unidentified	−	−	−	601.4	136.759	1.221
37	未知物7	unidentified	−	−	−	610.6	139.247	1.567
38	未知物8	unidentified	−	−	−	589	133.441	1.591
39	未知物9	unidentified	−	−	−	611.6	139.524	1.290
40	未知物10	unidentified	−	−	−	576.7	130.123	1.139
41	未知物11	unidentified	−	−	−	500.3	109.498	1.169
42	未知物12	unidentified	−	−	−	520.4	114.924	1.266
43	未知物13	unidentified	−	−	−	599.7	136.309	1.321
44	未知物14	unidentified	−	−	−	742	181.951	0.961
45	未知物15	unidentified	−	−	−	604.8	137.705	0.948
注：M_W表示化合物分子量；RI表示保留指数；RT表示保留时间（s）；DT表示漂移时间（ms）；M表示化合物单体；D表示化合物的二聚体。

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表 3 不同产地芋头中差异挥发性化合物

Table 3. Volatile differential compounds in taro from different origins

序号	化合物名称	CAS#	VIP	香气描述^a
1	未知物13	−	2.754
2	己酸甲酯-M	C106-70-7	2.631	果香味
3	异丙醇	C67-63-0	2.147	酒味
4	未知物6	−	1.992
5	辛酸甲酯	C111-11-5	1.659	蜡质味
6	未知物14	−	1.586
7	未知物15	−	1.570
2'	己酸甲酯-D	C106-70-7	1.506	果香味
8	2-甲基-乙酸丁酯	C624-41-9	1.173	果香味
9	未知物9	−	1.160
10	2-庚酮	C110-43-0	1.070	奶酪味
注：“−”表示无，^a表示香气描述参考http://www.thegoodscentscompany.com/search2.html。

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