羧甲基纳米纤维素稳定的低油相Pickering乳液凝胶的制备及性质分析

崔颂; 张月; 刘超然; 王一凡; 代蕾

doi:10.13386/j.issn1002-0306.2022070180

羧甲基纳米纤维素稳定的低油相Pickering乳液凝胶的制备及性质分析

doi: 10.13386/j.issn1002-0306.2022070180

青岛农业大学食品科学与工程学院，山东青岛 266109

基金项目: 山东省自然科学基金（ZR2020QC218）；青年人才托举工程（YESS20210248）。

详细信息

作者简介:
崔颂（1999−），女，硕士研究生，研究方向：食品科学与工程，E-mail：cuisong2521@163.com

通讯作者:
代蕾（1988−），女，博士，教授，研究方向：食品科学，E-mail：dailei508@163.com

中图分类号: TS201.7
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出版历程
- 收稿日期: 2022-07-19
- 刊出日期: 2023-05-15

Preparation and Property Analysis of Carboxymethyl Nanocellulose Stabilized Low Oil Phase Pickering Emulsion Gels

College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China

摘要

摘要: 结合pH对羧甲基纳米纤维素（CMCN）的ζ-电位、接触角的影响，本文以CMCN溶液为连续相，玉米油为分散相构建低油相（20%）Pickering乳液凝胶，研究了CMCN稳定的Pickering乳液凝胶的类型、微观结构、流变学特性和不同条件下的稳定性。结果表明：从pH2到pH9，CMCN的ζ-电位从−2 mV减小到−67 mV，其中在pH4时ζ-电位绝对值相对较大且具有最接近90°的接触角，因此pH4时的CMCN更适合稳定低油相Pickering乳液凝胶。荧光显微镜和流变学结果分别证明CMCN稳定的Pickering乳液凝胶为O/W型，且在低油浓度下形成了类弹性结构（G'>G''），为假塑性流体。低油相Pickering乳液凝胶贮藏120 d后仍可保持稳定。在1.3%以上浓度85 ℃加热和pH4以上CMCN稳定的乳液凝胶无分层现象且液滴尺寸变化较小证明其具有优异的pH和热稳定性。本研究制备的低油相Pickering乳液凝胶为绿色新型食品的开发提供了一种新思路，有望解决高油乳液凝胶不利于身体健康的问题。
- 羧甲基纳米纤维素（CMCN） /
- Pickering乳液凝胶 /
- 低油 /
- 流变学 /
- 稳定性
Abstract: Combined with the effect of pH on the ζ-potential and contact angle of carboxymethyl nanocellulose (CMCN), low oil phase (20%) Pickering emulsion gels were constructed with CMCN solution as the continuous phase and corn oil as the dispersant phase. The types, microstructure, rheological properties, and stability of CMCN-stabilized Pickering emulsion gels under different conditions were studied, respectively. The results showed that the ζ-potential of CMCN decreased from −2 mV to −67 mV when pH2 to pH9, the absolute value of the ζ-potential was relatively large at pH4 and the contact angle was closest to 90°. Therefore, CMCN at pH4 was more suitable for stabilizing low-oil phase Pickering emulsion gels. Fluorescence microscopy and rheological results showed that the CMCN-stabilized Pickering emulsion gels were O/W type, and the elastic structure (G'>G'') was formed at low oil concentration, which was pseudoplastic fluid. The low oil phase Pickering emulsion gels remained stable after 120 days of storage. The emulsion gels stabilized by CMCN at a concentration above 1.3% at 85 ℃ and above pH4 had no delamination and small droplet size change, which proved that the emulsion gels had excellent pH and thermal stability. The low oil phase Pickering emulsion gels prepared in this study would provide a new idea for the development of new green food, which was expected to solve the problem that high oil emulsion gels were not conducive to health.
- carboxymethyl nanocellulose (CMCN) /
- Pickering emulsion gels /
- low oil phase /
- rheology /
- stability

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图 1 CMCN的TEM图

Figure 1. TEM image of CMCN

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图 2 不同pH的CMCN的ζ-电位

注：不同字母表示差异显著，P<0.05；图5、图7同。

Figure 2. ζ-Potential of CMCN at different pH

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图 3 不同pH的CMCN的接触角

Figure 3. Contact angle of CMCN with different pH

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图 4 CMCN稳定Pickering乳液凝胶的荧光显微镜图像

Figure 4. Fluorescence microscope image of CMCN stabilized Pickering emulsion gels

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图 5 不同浓度的CMCN稳定的Pickering乳液凝胶的液滴尺寸和ζ-电位

注：A为液滴尺寸测量值；B为ζ-电位测量值。

Figure 5. Droplet size and ζ-potential of the Pickering emulsion gels stabilized with different concentrations of CMCN

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图 6 不同浓度的CMCN稳定Pickering乳液凝胶的流变学特性

注：A为黏度扫描；B为频率扫描。

Figure 6. Rheological properties of CMCN stabilized Pickering emulsion gels with different concentrations

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图 7 不同pH CMCN稳定的Pickering乳液凝胶的液滴尺寸图及外观

Figure 7. Droplet size and appearance of Pickering emulsion gels stabilized with different pH CMCN

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图 8 不同浓度CMCN的稳定的Pickering乳液凝胶的外观图及液滴尺寸

注：A为新鲜制备的Pickering乳液凝胶；B为室温下放置120 d后的Pickering乳液凝胶；C为Pickering乳液凝胶的液滴尺寸测量结果；不同小写字母为初乳差异显著（P<0.05）；不同大写字母为贮藏120 d后差异显著（P<0.05）。

Figure 8. Appearance and droplet size of Pickering emulsion gels stabilized with different concentrations of CMCN

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图 9 Pickering乳液凝胶的热稳定性

注：A为不同加热温度；B为加热不同浓度。字母不同的值差异有统计学意义（P<0.05），不同大小写字母代表不同样品。

Figure 9. Thermal stability of Pickering emulsion gels

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表 1 不同pH的CMCN的接触角

Table 1. Contact angle of CMCN at different pH values

pH	pH2	pH3	pH4	pH5	pH6	pH7	pH8	pH9
θ_o/w	47.65±0.75^a	61.30±0.30^b	66.25±1.25^b	57.10±6.90^ab	63.45±7.35^b	65.05±0.25^b	65.85±0.75^b	59.70±3.60^ab
注：同一行上标字母不同表示差异有统计学意义（P<0.05）。

下载: 导出CSV

表 2 不同CMCN浓度的Pickering乳液凝胶的流变学参数

Table 2. Rheological parameters of Pickering emulsion gels with different CMCN concentrations

CMCN浓度	1.0%	1.1%	1.2%	1.3%	1.4%
K	17.44948	23.73159	33.88483	34.65759	53.44787
n	0.30829	0.24731	0.23365	0.2105	0.18148
R²	0.99934	0.99951	0.99935	0.99918	0.99914

下载: 导出CSV

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