Inhibition of Starter Culture on Nitrite and Biogenic Amines in Fermented Duck Sausage
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摘要: 为探究发酵剂对鸭肉发酵香肠亚硝酸盐及生物胺的抑制作用,并为发酵香肠产品的开发及其安全性控制提供理论依据。本研究将发酵乳杆菌RA3和植物乳杆菌RC4以单一菌种或复配菌种接种于鸭肉发酵香肠,以自然发酵组作对照,测定香肠的亚硝酸盐、生物胺及其他理化指标。结果表明:在鸭肉发酵香肠加工贮藏过程中,各组的菌落总数及实验组生物胺含量总体上均呈现先上升后下降的趋势,各组的挥发性盐基氮(TVB-N)含量及自然组生物胺含量逐渐上升,亚硝酸盐含量则呈现下降趋势。实验组pH、TVB-N及亚硝酸盐含量始终低于自然组,在第28 d时RF组亚硝酸盐含量比自然组低1.33 mg/kg。在21~28 d,实验组的菌落总数显著低于自然组(P<0.05)。因此,接种发酵剂可以在一定程度上减少菌落总数,显著抑制亚硝酸盐和生物胺的形成(P<0.05),可以提高香肠的安全性。Abstract: In order to explore the inhibition of starter on nitrite and biogenic amines in duck fermented sausage, and provide a theoretical basis for the development and safety control of fermented sausage products. Lactobacillus fermentum RA3 and Lactobacillus plantarum RC4 were inoculated into duck fermented sausage with single or compound strains, and compared with the natural fermentation group, the nitrite, biogenic amines and other physicochemical indexes of the sausage were determined. The results showed that during the processing and storage of sausage, the total number of bacterial colony and the content of biogenic amines in the experimental group increased first and then decreased. While the content of volatile basic nitrogen (TVB-N) and biogenic amine in natural group increased gradually. The content of nitrite showed a downward trend. In addition, the pH, TVB-N and nitrite content were always lower than those in the natural group. On the 28th day, the nitrite content of the RF group was 1.33 mg/kg lower than that of the natural group. From the day 21 to 28, the total number of colonies in the experimental group was significantly lower than that in natural group (P<0.05). In conclusion, inoculating starter could effectively reduce the total number of colonies to a certain extent, while the formation of nitrite and biogenic amines was significantly inhibited (P<0.05), thereby improving the safety of sausage.
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Key words:
- duck fermented sausage /
- starter /
- safety /
- nitrite /
- biogenic amines
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表 1 不同发酵剂对发酵香肠亚硝酸盐含量的影响
Table 1. Influence of different starter on nitrite content in fermented sausage
时间(d) 含量(mg/kg) 自然组 RA RC RF 0 21.44±0.07Cc 15.04±0.18Cb 14.80±0.26Db 11.53±0.03Ca 7 18.04±0.06Bb 10.15±0.26Ba 9.96±0.18Ca 9.90±0.22Ba 14 10.54±0.14Ac 10.01±0.12Bb 9.73±0.17BCa 9.74±0.07Ba 21 10.64±0.16Ad 9.11±0.11Aa 9.45±0.03ABb 9.66±0.10Bc 28 10.53±0.10Ac 9.90±0.07Bb 9.39±0.16Aa 9.20±0.14Aa 表 2 不同发酵剂对发酵香肠生物胺含量的影响
Table 2. Influence of different starter on biogenic amine content of fermented sausage
生物胺含量(mg/kg) 贮藏期(d) 0 7 14 21 28 色胺 自然组 5.28±0.23Aa 15.61±0.56Bc 15.74±0.09Bab 16.10±0.72Bd 36.73±0.72Cd RA 6.72±0.28Ab 8.42±0.20Ba 16.82±0.15Cb 8.50±0.19Ba 8.36±0.13Bc RC 7.74±0.13Bc 16.28±0.16Dc 33.30±0.69Ec 12.19±0.17Cc 5.63±0.05Ab RF 9.72±0.20Bd 14.28±0.16Cb 15.04±0.56Ca 10.65±0.58Bb 3.30±0.05Aa 苯乙胺 自然组 4.46±0.16Aa 5.86±0.13Ba 7.54±0.59Cb 8.67±0.41Dc 11.37±0.55Ec RA 5.28±0.43BCa 5.62±0.43Ca 5.86±0.57Ca 4.32±0.20Ba 3.12±0.19Aa RC 5.12±0.15ABa 7.19±0.23Cb 11.58±0.71Dc 6.40±0.37BCb 4.80±0.73Ab RF 4.55±0.34Aa 6.95±0.32Bb 8.02±0.47Bb 4.52±0.72Aa 3.38±0.27Aa 腐胺 自然组 0.00±0.00Aa 4.24±0.20Ba 4.54±0.37Ba 18.64±0.73Cc 49.10±1.42Db RA 5.12±0.13Bc 6.62±0.37Cc 7.44±0.23Db 5.40±0.30Ba 4.26±0.30Aa RC 3.25±0.23Ab 5.50±0.35Bb 7.86±0.43Cb 7.44±0.26Cb 3.08±0.06Aa RF 3.72±0.22Bb 7.26±0.50Dc 7.70±0.56Db 5.78±0.47Ca 2.48±0.42Aa 尸胺 自然组 2.06±0.08Aa 2.54±0.47Aa 4.31±0.18Ba 4.38±0.23Ba 6.83±0.44Cc RA 2.28±0.01Ab 4.05±0.01Bc 5.62±0.37Cb 4.56±0.36Ba 4.07±0.08Bb RC 2.82±0.01Bd 3.80±0.01Cbc 4.76±0.27Da 4.67±0.20Da 2.32±0.13Aa RF 2.62±0.01Ac 3.36±0.01Bb 4.44±0.27Ca 4.32±0.29Ca 2.46±0.19Aa 组胺 自然组 3.48±0.20Ab 3.60±0.35Aa 5.32±0.19Bb 5.66±0.35Bc 7.33±0.48Cc RA 2.46±0.29Aa 3.36±0.22Ba 5.31±0.24Cb 3.44±0.13Ba 3.12±0.13Bb RC 4.40±0.22Bc 4.56±0.28Bb 4.38±0.34Ba 4.46±0.15Bb 3.12±0.13Ab RF 3.26±0.13Bb 4.36±0.23Cb 8.72±0.30Ec 5.50±0.19Dc 2.46±0.26Aa 酪胺 自然组 8.49±0.16Aa 8.62±0.36Aa 12.22±0.23Ba 12.49±0.20Bc 12.54±0.16Bb RA 8.72±0.13Aab 11.84±0.36Cc 14.70±0.30Dc 10.32±0.36Bb 9.38±0.33Aa RC 9.17±0.37Abc 12.85±0.01Bd 15.19±0.20Cc 9.20±0.23Aa 8.82±0.20Aa RF 9.68±0.13Bc 10.74±0.13Cb 13.31±0.20Db 9.77±0.51Bab 8.88±0.30Aa 亚精胺 自然组 1.23±0.03Ac 1.64±0.30ABc 2.16±0.12Bb 6.50±0.22Cd 7.50±0.32Db RA 0.00±0.00Aa 0.14±0.01Ba 1.12±0.04Da 0.58±0.05Cb 0.18±0.01Ba RC 0.08±0.01Ab 1.04±0.01Bb 2.22±0.06Cb 0.12±0.01Aa 0.07±0.01Aa RF 0.00±0.00Aa 2.06±0.06Cc 3.26±0.16Dc 1.93±0.16Cc 0.32±0.02Ba 精胺 自然组 0.18±0.01Aa 0.37±0.01Ba 1.55±0.04Cab 2.44±0.12Dd 3.72±0.06Ed RA 0.95±0.01Cc 1.08±0.09Cc 1.4±0.08Da 0.66±0.15Ba 0.40±0.01Aa RC 0.96±0.01ABc 1.48±0.13Cd 1.68±0.06Db 1.1±0.01Bb 0.87±0.01Ac RF 0.32±0.02Ab 0.76±0.06Bb 2.62±0.15Dc 2.08±0.08Cc 0.70±0.04Bb 总生物胺 自然组 25.18±0.87Aa 42.48±2.38Bb 53.38±1.81Ca 74.88±2.98Dd 135.12±4.15Ed RA 31.53±1.28Ab 41.13±1.69Da 58.27±1.98Eb 37.78±1.74Ca 32.89±1.18Bc RC 33.54±1.13Bc 52.70±1.18Dd 80.97±2.76Ed 45.58±1.40Cc 28.71±1.32Ab RF 33.87±1.05Bd 49.77±1.47Dc 63.11±2.67Ec 44.55±3.00Cb 23.98±1.55Aa -
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