小米制品对番泻叶致小鼠腹泻的缓解作用

陈树俊; 郝民权; 张丽珍

doi:10.13386/j.issn1002-0306.2022080022

小米制品对番泻叶致小鼠腹泻的缓解作用

doi: 10.13386/j.issn1002-0306.2022080022

山西大学生命科学学院，山西太原 030006

基金项目: 国家重点研发计划（2020YFD1001405）；“特优”农产品功能化加工关键技术研究与应用（202102140601014）。

详细信息

作者简介:
陈树俊（1964−），男，本科，教授，研究方向：食品新工艺与功能食品，E-mail：chenshujun515@163.com

通讯作者:
张丽珍（1977−），女，博士，教授，研究方向：生物资源筛选及利用，E-mail：lizhen@sxu.edu.cn

中图分类号: TS210.4
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出版历程
- 收稿日期: 2022-08-03
- 刊出日期: 2023-05-15

Alleviating Effect of Millet Products on Diarrhea Induced by Senna Leaf in Mice

College of Life Science, Shanxi University, Taiyuan 030006, China

摘要

摘要: 探讨不同小米制品对番泻叶致小鼠腹泻的缓解作用。84只昆明小鼠随机分为7组：正常对照组、模型组、阳性对照组、发酵小米组（6.16 g/kg）、发酵米糠组（2.05 g/kg）、小米粥油组（10.27 g/kg）、米糠粥油组（10.27 g/kg）。采用番泻叶建立小鼠腹泻模型，造模7 d后，除模型组外，其它各组开始为期7 d的治疗，以腹泻指数、脏器指数、血清炎症因子、分泌型免疫球蛋白A（secretory immunoglobulin A，SIgA）水平为指标，并通过小肠切片观察来评价小米制品对腹泻小鼠的影响。结果表明，与模型组相比，小米制品组第13 d小鼠体重显著增加（P<0.05），腹泻指数显著降低（P<0.05），发酵米糠、发酵小米和小米粥油组血清炎症因子含量显著降低（P<0.05），肠粘膜分泌型免疫球蛋白A含量显著升高（P<0.05），小肠组织切片观察完整程度较好。小米制品中发酵米糠、发酵小米和小米粥油对番泻叶诱导小鼠腹泻有一定的缓解作用，但治疗作用机制及肠道菌群变化仍有待进一步探讨。
- 小米 /
- 发酵 /
- 发酵米糠 /
- 小米粥油 /
- 米糠粥油 /
- 抗腹泻 /
- 炎性因子 /
- 肠粘膜
Abstract: To study the alleviating effect of different millet products on diarrhea caused by senna leaf in mice. Eighty-four KM mice were randomly divided into 7 groups: Normal control group, model group, positive control group, fermented millet group (6.16 g/kg), ferment millet bran group (2.05 g/kg), millet gruel oil group (10.27 g/kg), and millet bran porridge oil group (10.27 g/kg). Senna leaf was used to establish a diarrhea model in mice. After 7 days of modeling, except for the model group, the other groups were treated for 7 days. The diarrhea index, viscera index, serum inflammatory factors, secretory immunoglobulin A (SIgA) levels, and small intestine section observation were used to evaluate the effects of millet products on diarrhea mice. Compared with the model group, the weight of mice in the millet product group increased significantly on the 13th day (P<0.05), the diarrhea index was significantly decreased (P<0.05), the contents of serum inflammatory factors in ferment millet bran group, fermented millet group, and millet porridge oil group were significantly decreased (P<0.05), the content of secreted immunoglobulin A in intestinal mucosa was significantly increased (P<0.05), small intestine tissue section observation integrity was good. Fermented millet bran, fermented millet, and millet porridge oil in millet products could alleviate diarrhea induced by senna leaf in mice, but the therapeutic mechanism and the changes of intestinal flora in them remain to be further explored.
- millet /
- fermentation /
- fermented millet bran /
- millet gruel oil /
- millet bran porridge oil /
- anti-diarrhea /
- inflammatory factor /
- intestinal mucosa

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图 1 实验过程中各组小鼠体重变化

Figure 1. Body weight changes of mice in each group during the experiment

下载: 全尺寸图片幻灯片

图 2 小米制品对小鼠血清炎症因子表达的影响

注：小写字母完全不同表示具有显著性差异，P<0.05；图3~图4同。

Figure 2. Effects of millet products on the expression of serum inflammatory factors in mice

下载: 全尺寸图片幻灯片

图 3 小米制品对小鼠脾脏和胸腺脏器指数的影响

Figure 3. Effect of millet products on spleen and thymus organ index in mice

下载: 全尺寸图片幻灯片

图 4 小米制品对小鼠肠粘膜sIgA含量的影响

Figure 4. Effect of millet products on sIgA content in intestinal mucosa of mice

下载: 全尺寸图片幻灯片

图 5 不同处理组小肠切片H&E染色代表性图像（200倍显微镜观测）

注：A.空白对照组；B.模型对照组；C.阳性对照组；D.发酵小米组；E.发酵小米糠组；F.小米粥油组； G.小米糠粥油组。

Figure 5. Representative images of H&E staining in small intestine sections of different treatment groups （200×microscope）

下载: 全尺寸图片幻灯片

表 1 动物实验分组及灌胃剂量

Table 1. Grouping and process of animal experiments

组别	造模期（7 d）	治疗期（7 d）
正常对照组（NC）	生理盐水（20 mL/kg）	生理盐水（20 mL/kg）
模型对照组（MC）	番泻叶水煎剂（1 g/mL，20 mL/kg）	生理盐水（20 mL/kg）
阳性对照组（PC）	番泻叶水煎剂（1 g/mL，20 mL/kg）	盐酸洛哌丁胺悬液（0.2 g/mL，20 mL/kg）
发酵小米组（FM）	番泻叶水煎剂（1 g/mL，20 mL/kg）	发酵小米提取液冻干粉溶液（6.16 g/kg）
发酵米糠组（FMB）	番泻叶水煎剂（1 g/mL，20 mL/kg）	发酵米糠提取液冻干粉溶液（2.05 g/kg）
小米粥油组（MGO）	番泻叶水煎剂（1 g/mL，20 mL/kg）	小米粥油冻干粉溶液（10.27 g/kg）
米糠粥油组（MBPO）	番泻叶水煎剂（1 g/mL，20 mL/kg）	米糠粥油冻干粉溶液（10.27 g/kg）

下载: 导出CSV

表 2 小鼠腹泻指数的变化

Table 2. Changes of diarrhea index in mice

组别	腹泻指数
组别	第7 d	第10 d	第13 d
正常对照组（NC）	0^b	0^e	0^d
模型对照组（MC）	2.04±0.43^a	1.67±0.25^a	0.84±0.14^a
阳性对照组（PC）	1.67±0.24^a	0.84±0.15^d	0.21±0.08^cd
发酵小米组（FM）	1.78±0.13^a	1.14±0.11^bcd	0.36±0.19^bc
发酵米糠组（FMB）	1.70±0.27^a	1.28±0.26^bc	0.40±0.11^bc
小米粥油组（MGO）	1.65±0.14^a	0.97±0.15^cd	0.38±0.18^bc
米糠粥油组（MBPO）	1.88±0.18^a	1.41±0.22^ab	0.54±0.14^b
注：同一列中，小写字母完全不同表示具有显著性差异，P<0.05。

下载: 导出CSV

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