藻蓝蛋白对环磷酰胺致小鼠肝肾损伤的保护作用

李新月; 赵猛; 丁子康; 王晓梅; 王瑞俊; 张忠山

doi:10.13386/j.issn1002-0306.2022080069

藻蓝蛋白对环磷酰胺致小鼠肝肾损伤的保护作用

doi: 10.13386/j.issn1002-0306.2022080069

湖州师范学院生命科学学院，浙江省水生生物资源养护与开发技术研究重点实验室，浙江湖州 313000

基金项目: 湖州师范学院研究生科研创新项目（2022KYCX60）；浙江省基础公益计划项目（LGN21D060001）；湖州市自然科学基金（2020YZ06）。

详细信息

作者简介:
李新月（1996−），女，硕士研究生，研究方向：水产品加工与贮藏，E-mail：Lixinyue_Z0601@163.com

通讯作者:
张忠山（1982−），男，博士，教授，研究方向：水产品加工与贮藏，E-mail：01959@zjhu.edu.cn

中图分类号: TS254.9
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出版历程
- 收稿日期: 2022-08-08
- 刊出日期: 2023-05-15

Protective Effect of Phycocyanin on Cyclophosphamide-induced Immunocompromised Mice

Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, China

摘要

摘要: 目的：研究藻蓝蛋白对环磷酰胺诱导小鼠肝肾组织损伤的保护作用，为评价肝肾治疗药物提供新的研究思路。方法：雌性BALB/c小鼠随机分为空白组、模型组、阳性对照组以及藻蓝蛋白低、中、高剂量组。采用腹腔注射环磷酰胺后建立小鼠肝肾损伤模型，然后空白组和模型组小鼠灌胃生理盐水，样品组给予不同剂量藻蓝蛋白溶液（50、100、200 mg/kg），阳性对照组灌胃盐酸左旋咪唑（40 mg/kg），灌胃时间5 d。采用试剂盒法分别测定各组小鼠血清中的白细胞介素-2（IL-2）、肿瘤因子-α（TNF-α）和免疫球蛋白（IgG）、超氧化物歧化酶（SOD）、丙二醛（MDA）水平；肝组织的超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）、天冬氨酸氨基转移酶（AST）、谷丙转氨酶（ALT）；及肾组织的超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）、尿酸（UA）、尿素氮（BUN）水平并观察小鼠肝脏、肾脏组织形态学变化。结果：与模型组对比，藻蓝蛋白剂量组小鼠血清中的IL-2、TNF-α、MDA水平极显著下降（P<0.01）；藻蓝蛋白高剂量组可明显降低血清中的IgG水平（P<0.05）并提高血清中的SOD水平（P<0.05）；同时可降低由环磷酰胺引起的小鼠血清中MDA含量升高的情况（P<0.01），升高SOD活性（P<0.05）；此外，藻蓝蛋白剂量组小鼠的肝组织GSH-Px水平极显著高于模型组（P<0.01），而MDA、ALT、AST水平均极显著低于模型组小鼠（P<0.01）；相较于肝组织，藻蓝蛋白剂量组小鼠肾组织的MDA水平极显著低于模型组（P<0.01），仅低剂量藻蓝蛋白组小鼠肾组织的BUN水平极显著低于模型组（P<0.01），高剂量藻蓝蛋白组小鼠肾组织的UA水平极显著低于模型组（P<0.01）。结论：综合各项指标结果，藻蓝蛋白对环磷酰胺导致的小鼠肝肾损伤具有明显改善作用。
- 藻蓝蛋白 /
- 环磷酰胺 /
- 肝肾损伤 /
- 保护作用
Abstract: Objective: To investigate the protective effect of phycocyanin on hepatorenal co-damage in mice caused by cyclophosphamide, and to provide new research ideas for evaluating liver and kidney therapy drugs. Methods: Female BALB/c mice were randomly divided into blank group, model group, positive control group, high dose group, middle dose group and low dose group. The hepatic and kindy injury model of mice were established by intraperitoneal injection of cyclophosphamide (50, 100, 200 mg/kg), then the mice of blank group and model group were fed with normal saline, mice of positive control group were fed levimidazole hydrochloride (40 mg/kg) and the mice of dosage groups were fed different dosage of instant power of phycocyanin. Interleukin-2 (IL-2), tumournecrosis factor-α (TNF-α), immunoglobulin (IgG), superoxide dismutase (SOD), malondialdehyde (MDA) of serum in mice, superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) level of liver tissue and superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), uric acid (UA), urea nitrogenin (BUN) level of kindey tissue in each group mice were determined by reagent kit method. Experimental results showed that compared with the model group, the serum levels of IL-2, TNF-α and MDA in mice in the phycocyanin dose group decreased significantly (P<0.01). The high-dose group of phycocyanin significantly reduced serum IgG levels (P<0.05) and increased serum SOD levels (P<0.05). At the same time, it could reduce the elevated content of MDA in mice caused by cyclophosphamide (P<0.01) and increase SOD activity (P<0.05). In addition, the GSH-Px levels of liver tissue in mice in the phycocyanin dose group were significantly higher than those in the model group (P<0.01), while the levels of MDA, ALT, and AST were significantly lower than those in the model group (P<0.01). Compared with liver tissue, the MDA level of mouse kidney tissue in the phycocyanin dose group was significantly lower than that in the model group (P<0.01), the BUN level of mouse kidney tissue in the low-dose phycocyanin group alone was significantly lower than that in the model group (P<0.01), and the UA level of mouse kidney tissue in the high-dose phycocyanin group was significantly lower than that in the model group (P<0.01). So the powder of phycocyanin had protective effect on cyclophosphamide-induced hepatic and kindy injury in mice.
- phycocyanin /
- cyclophosphamide /
- liver and kidney injury /
- protection

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图 1 小鼠肝脏组织病理学变化（H&E染色，100×）

Figure 1. Histopathological changes of mouse liver (H&E staining, 100×)

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图 2 小鼠肾脏组织病理学变化（H&E染色，100×）

Figure 2. Histopathological changes of mouse kidney (H&E staining, 100×)

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图 3 PC对小鼠血清中SOD、MDA活力的影响

注：与空白组CK相比，##表示P<0.01；与模型组M相比，*表示P<0.05，**表示P<0.01；图4~图6同。

Figure 3. Effect of PC on the activity of SOD and MDA in mouse serum

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图 4 PC对CTX致免疫低下小鼠血清免疫因子水平的影响（n=6）

Figure 4. Effect of PC on serum immune factor level of CTX induced immunosuppressive mice (n=6)

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图 5 PC对CTX致免疫低下小鼠肝（A、B、C）、肾（D、E、F）抗氧化酶水平的影响（n=6）

Figure 5. Effect of PC on the level of antioxidant enzymes in liver (A, B, C) and kidney (D, E, F) of CTX induced immunosuppressive mice (n=6)

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图 6 PC对CTX致免疫低下小鼠肝（A、B）、肾（C、D）特异性功能水平的影响（n=6）

Figure 6. Effect of PC on liver (A, B) and kidney (C, D) specific function level of CTX induced immunosuppression mice (n=6)

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表 1 PC对CTX致免疫低下小鼠免疫脏器指数的影响（n=6）

Table 1. Effect of PC on immune organ index of CTX induced immunosuppressive mice (n=6)

组别	解剖前体重（g）	胸腺指数（%）	脾脏指数（%）	肝脏指数（%）	肾脏指数（%）
CK	20.0667±0.3559	0.0026±0.0002	0.0035±0.0001	0.0482±0.0009	0.0138±0.0004
M	17.8167±0.8495^##	0.0025±0.0003	0.0035±0.0001	0.0473±0.0014	0.0135±0.0005
LH	20.4000±0.3847	0.0035±0.0007^**	0.0044±0.0003^**	0.0457±0.0013	0.0138±0.0003
ZL	20.4167±1.000	0.0038±0.0002^**	0.0049±0.0004^**	0.0449±0.0009	0.0139±0.0005
ZM	20.4000±1.0564	0.0037±0.0006^**	0.0050±0.0003^**	0.0458±0.0015	0.0136±0.0008
ZH	20.0500±0.7450	0.0039±0.0005^**	0.0052±0.0005^**	0.0455±0.0015	0.0135±0.0004
注：与空白组CK相比，^##P<0.01；与模型组M相比，^**P<0.01。

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