中国毛虾活性肽对免疫抑制小鼠免疫调节作用的影响

杨志艳; 惠婷婷; 祝宝华; 徐晨晨; 李燕; 李晓晖

doi:10.13386/j.issn1002-0306.2022080148

中国毛虾活性肽对免疫抑制小鼠免疫调节作用的影响

doi: 10.13386/j.issn1002-0306.2022080148

杨志艳^1,,
惠婷婷¹,
祝宝华¹,
徐晨晨¹,
李燕^{1, 2, 3},
李晓晖^{1, 2, 3,*, ,}

1.
上海海洋大学食品学院，上海 201306
2.
上海水产品加工及贮藏工程技术研究中心，上海 201306
3.
农业部水产品贮藏保鲜质量安全风险评估实验室（上海），上海 201306

基金项目: 国家重点研发计划（低值水产品及副产物高值化利用与新产品创制，2019YFD0902000）。

详细信息

作者简介:
杨志艳（1994−），女，硕士研究生，研究方向：生物活性肽功能，E-mail：2447231397@qq.com

通讯作者:
李晓晖（1975−），女，博士，副教授，研究方向：食品微生物，E-mail：xhli@shou.edu.cn

中图分类号: TS254.1
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- 文章访问数: 18
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- 被引次数: 0
出版历程
- 收稿日期: 2022-08-15
- 刊出日期: 2023-05-01

Effects of Peptides from Acetes chinensis on Immunoregulation in Immunocompromised Mice

YANG Zhiyan^1
,,
HUI Tingting¹,
ZHU Baohua¹,
XU Chenchen¹,
LI Yan^{1, 2, 3},
LI Xiaohui^{1, 2, 3,*
, ,}

1.
College of Food Science and Tecnology, Shanghai Ocean University, Shanghai 201306, China
2.
Shanghai Aquatic Product and Processing and Storage Engineering Technology Research Center, Shanghai 201306, China
3.
Laboratory for Risk Assessment of Quality and Safety of Storage and Preservation of Aquatic Products, Ministry of Agriculture, Shanghai 201306, China

摘要

摘要: 本文以毛虾为原料，采用蛋白酶酶解获得毛虾活性肽，通过建立免疫抑制小鼠模型探究了毛虾活性肽对免疫抑制小鼠的免疫调节功能。小鼠随机分为六组：正常对照组、模型组、阳性对照组以及低、中、高剂量组，其中剂量组分别灌胃0.25 g·kg⁻¹ BW、0.5 g·kg⁻¹ BW、1.0 g·kg⁻¹ BW毛虾活性肽。结果表明，剂量组免疫抑制小鼠体重明显增加（P<0.05），免疫器官指数中的胸腺指数显著提高（P<0.05），此外，毛虾活性肽还促进了小鼠血清中免疫球蛋白（IgA、IgG和IgM）以及细胞因子（IL-2、IL-6和TNF-α）水平的提升（P<0.05），外周血白细胞总数基本恢复至正常水平，NK细胞活性也显著增强（P<0.05）。综上，毛虾活性肽具有改善免疫抑制剂导致的免疫功能损伤，增强免疫抑制小鼠免疫调节的作用。
- 中国毛虾 /
- 酶解 /
- 免疫抑制小鼠 /
- 免疫调节
Abstract: In this paper, the active peptides were obtained from Acetes chinensis with protease, and then the immunoregulatory function and mechanism of them on the immunosuppressed mice were explored by establishing an immunosuppressive mouse model. Mice were randomly divided into six groups: Normal control group, model group, positive control group and low, medium and high dose groups, among which, the dose groups were given 0.25 g· kg⁻¹ BW, 0.5 g·kg⁻¹ BW, 1.0 g·kg⁻¹ BW A. chinensis active peptide. The results showed that the body weight of the immunosuppressed mice in dosegroups was significantly increased (P<0.05), and the thymus index of the immune organ indexes were remarkably improved (P<0.05) after being stimulated by the active peptides of Acetes chinensis. The serum levels of immunoglobulins (IgA, IgG, and IgM) and cytokines (IL-2, IL-6, and TNF-α) in mice were enhanced (P<0.05), and the total number of peripheral blood leukocytes was increased. In addition, NK cells activity was also significantly enhanced (P<0.05). To sum up, the active peptides of A. chinensis could improve the immune function damage caused by immunosuppression, and the immunoregulatory function of immunosuppressed mice.
- Acetes chinensis /
- enzymatic hydrolysis /
- immunosuppressed mice /
- immunoregulation

HTML全文

图 1 毛虾活性肽对免疫抑制小鼠分泌免疫球蛋白IgM（a）、IgA（b）、IgG（c）的影响

Figure 1. Effects of the active peptide of A. chinensis on the secretion of immunoglobulin IgM (a), IgA (b) and IgG (c) in immunosuppressed mice

下载: 全尺寸图片幻灯片

图 2 毛虾活性肽对免疫抑制小鼠分泌免疫球蛋白TNF-α（a）、IL-2（b）、IL-6（c）的影响

Figure 2. Effects of the active peptide of A. chinensis on the secretion of immunoglobulin TNF-α (a), IL-2 (b) and IL-6 (c) in immunosuppressed mice

下载: 全尺寸图片幻灯片

图 3 毛虾活性肽对免疫抑制小鼠NK细胞活力的影响

Figure 3. Effect of peptides from A. chinensis on the viability of immunosuppressed mice NK cells

下载: 全尺寸图片幻灯片

表 1 毛虾活性肽对免疫抑制小鼠体重和免疫器官指数的影响

Table 1. Effects of peptides from A. chinensis on the body weight and immune organ index in immunosuppressed mice

组别	初体重（g）	建模后体重（g）	终体重（g）	胸腺指数（mg·g⁻¹）	脾脏指数（mg·g⁻¹）
正常对照组	18.69±0.95	18.94±0.78	19.26±0.79	1.51±0.24	5.62±0.28
模型组	18.69±0.71	17.59±1.13^*	18.97±0.63	1.31±0.37^*	4.58±0.22
阳性对照组	18.91±0.60	17.47±1.02	19.38±0.62	1.69±0.26^##	5.44±0.27
低剂量组	18.95±0.95	17.72±1.28	19.75±0.78	1.67±0.23^##	5.43±0.47
中剂量组	17.90±0.53	16.79±0.83	19.93±0.37^#	1.57±0.23^#	5.17±0.55
高剂量组	18.32±0.65	17.21±1.18	20.32±0.92^#	1.69±0.44^##	5.00±0.76
注：、、**分别表示模型组与正常对照组比较具有统计学差异（P<0.05）、具有显著统计学差异（P<0.01）、具有极显著统计学差异（P<0.001）；#、##、###分别表示阳性对照组和剂量组与模型组比较具有统计学差异（P<0.05）、具有显著统计学差异（P<0.01）、具有极显著统计学差异（P<0.001）；Δ、ΔΔ、ΔΔΔ分别表示阳性与剂量组比较具有统计学差异（P<0.05）、具有显著统计学差异（P<0.01）、具有极显著统计学差异（P<0.001）；图1~图3、表2同。

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表 2 毛虾活性肽对免疫抑制小鼠外周血白细胞数量的影响

Table 2. Effect of peptides from A. chinensis on the number of peripheral blood leukocytes in immunosuppressed mice

组别	正常对照组	模型组	阳性对照组	低剂量组	中剂量组	高剂量组
白细胞数量（10⁹/L）	8.83± 0.22	6.56± 0.32^***	8.28± 0.45^##	8.59± 0.34^##	8.8± 0.56^##	8.69± 0.48^##

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