不同地区鲊广椒细菌多样性比较研究

杨晨; 柯悦; 黄锶钘; 刘梦琦; 张彦; 郭壮; 王玉荣

doi:10.13386/j.issn1002-0306.2022080269

不同地区鲊广椒细菌多样性比较研究

doi: 10.13386/j.issn1002-0306.2022080269

杨晨^{1, 2,},
柯悦^{1, 2},
黄锶钘^{1, 2},
刘梦琦^{1, 2},
张彦^{1, 3},
郭壮^{1, 2},
王玉荣^{1, 2,*, ,}

1.
湖北文理学院，湖北省食品配料工程技术研究中心，湖北襄阳 441053
2.
湖北文理学院，乳酸菌生物技术与工程襄阳市重点实验室，湖北襄阳 441053
3.
安琪纽特股份有限公司，酵母功能湖北省重点实验室，湖北宜昌 443003

基金项目: 襄阳市基础研究类科技计划项目（2021ABS002937）；湖北文理学院大学生创新创业训练计划项目（X202210519191）；湖北省自然科学基金计划联合基金项目（2022CFD008）。

详细信息

作者简介:
杨晨（2001−），女，大学本科，研究方向：食品生物技术，E-mail：3064657910@qq.com

通讯作者:
王玉荣（1993−），女，博士，讲师，研究方向：食品微生物与生物技术，E-mail：wangyurong1993@163.com

中图分类号: TS213.4
计量
- 文章访问数: 19
- HTML全文浏览量: 27
- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2022-08-30
- 刊出日期: 2023-05-15

Comparative Study on Bacterial Diversity of Zha-Chili in Different Regions

YANG Chen^{1, 2
,},
KE Yue^{1, 2},
HUANG Sixing^{1, 2},
LIU Mengqi^{1, 2},
ZHANG Yan^{1, 3},
GUO Zhuang^{1, 2},
WANG Yurong^{1, 2,*
, ,}

1.
Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang 441053, China
2.
Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory, Hubei University of Arts and Science, Xiangyang 441053, China
3.
Hubei Provincial Key Laboratory of Yeast Function, Angel Nutritech Co., Ltd., Yichang 443003, China

摘要

摘要: 本研究使用Illumina MiSeq测序技术解析兴山和保靖鲊广椒细菌群落结构。α多样性分析表明，兴山鲊广椒群落多样性及丰度均低于保靖鲊广椒（P<0.05）。两地鲊广椒中细菌主要是隶属于厚壁菌门（Firmicutes）的Lactobacillus（乳酸杆菌属），其在兴山鲊广椒中含量为50.24%，在保靖鲊广椒中为88.26%。β多样性分析发现，兴山和保靖鲊广椒细菌群落结构整体上具有显著差异（P<0.01），同时发现11个差异显著的细菌类群，其中得分较高的为Lactobacillus和Chishuiella（赤水河菌属）。表型预测发现，兴山鲊广椒细菌类群在可移动原件、生物膜形成、革兰氏阴性、致病潜力和氧化胁迫耐受等表型上表达显著，而保靖鲊广椒细菌类群则在革兰氏阳性表型上表达显著。
- 高通量测序 /
- 鲊广椒 /
- 群落结构 /
- 细菌多样性 /
- 表型预测
Abstract: In this study, Illumina MiSeq sequencing technology was used to analyze the bacterial community structure of Zha-Chili from Xingshan and Baojing. The α-diversity analysis showed that the community diversity and abundance of Zha-Chili in Xingshan were significantly lower than those in Baojing (P<0.05). Lactobacillus belonging to the Firmicutes was the main bacteria in the two places, the abundance of which was 50.24% in Xingshan and 88.26% in Baojing. β diversity analysis found that the bacterial community structure of Xingshan and Baojing Zha-Chili was significantly different on the whole (P<0.01) and 11 biomarkers were identified, among which Lactobacillus and Chishuiella were the most influential ones. Phenotypes prediction showed that bacterial groups of Zha-Chili of Xingshan were significantly expressed in movable elements, biofilm formation, gram negative, pathogenic potential and tolerance to oxidative stress, while the bacteria in Baojing Zha-Chili were significantly expressed in gram-positive.
- high-throughput sequencing /
- Zha-Chili /
- community structure /
- bacterial diversity /
- phenotypes prediction

HTML全文

图 1 香农和超1指数的箱形图

注：*表示差异显著（P<0.05），**表示差异非常显著（P<0.01），***表示差异极显著（P<0.001）；图5同。

Figure 1. Shannon and Chao 1 index box graph

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图 2 基于门水平的鲊广椒细菌多样性分析

Figure 2. Analysis of bacterial diversity of Zha-Chili based on phylum level

下载: 全尺寸图片幻灯片

图 3 基于属水平的鲊广椒细菌多样性分析

Figure 3. Bacterial diversity analysis of Zha-Chili based on genus level

下载: 全尺寸图片幻灯片

图 4 基于OTU水平的鲊广椒细菌类群结构分析

Figure 4. Analysis of bacterial group structure of Zha-Chili based on OTU level

下载: 全尺寸图片幻灯片

图 5 基于非加权的UniFrac 主坐标分析（a）和欧式距离分析（b）

Figure 5. Unweighted UniFrac principal coordinate analysis (a) and Euclidean distance analysis (b)

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图 6 鲊广椒细菌类群LDA值分布柱状图

Figure 6. Histogram of LDA value distribution of bacterial groups in Zha-Chili

下载: 全尺寸图片幻灯片

图 7 细菌类群的表型预测分析

Figure 7. Phenotypic prediction and analysis of bacterial groups

下载: 全尺寸图片幻灯片

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